Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Name: Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF
Brand: R&D Systems
SKU: 10095-KD

Catalog # 10095-KD | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P35918
Structure / Form:	Monomer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse VEGFR2/KDR/Flk-1 protein Ala20-Glu762, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala20
Predicted Molecular Mass	844 kDa
SDS-PAGE	844 kDa
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse VEGFR2/KDR/Flk-1 His-tag (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Mouse VEGF 164 (Catalog # 493-MV) that produces 50% of the optimal binding response is 0.015-0.12 μg/mL.

Scientific Data Examples for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

	Recombinant Mouse VEGFR2/Flk-1 His-tag Protein Binding Activity When Recombinant Mouse VEGFR2/KDR/Flk-1 (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Mouse VEGF 164 (Catalog # 493-MV) binds with an ED₅₀ of 0.015-0.12 μg/mL.
	Recombinant Mouse VEGFR2/Flk-1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse VEGFR2/KDR/Flk‑1 His-tag (Catalog # 10095-KD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-132 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10095-KD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and a tyrosine kinase domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743aa residue extracellular domain, a 21 aa residue transmembrane domain and a 584 aa residue cytoplasmic domain. The mature mouse VEGFR2 shares 80% and 93% sequence identity to human and rat VEGFR2. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 binds VEGF with high affinity and is a potent VEGF antagonist (1, 2).

Item	Value
References	Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Alternate Names	CD309, CD309 antigen, EC 2.7.10, EC 2.7.10.1, FLK1tyrosine kinase growth factor receptor, Fetal liver kinase 1, Flk-1, Flk1, KDR, KRD1, Kinase insert domain receptor, Ly73, Protein-tyrosine kinase receptor flk-1, VEGF R2, VEGFR, VEGFR-2, VEGFR2, fetal liver kinase-1, kinase insert domain receptor (a type III receptor tyrosine kinase), soluble VEGFR2, vascular endothelial growth factor receptor 2

Citations for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

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Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Catalog # 10095-KD | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Scientific Data Examples for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: VEGFR2/KDR/Flk-1

Citations for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Product Documents for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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