Recombinant Mouse PRDC/GREM2 Protein

Name: Recombinant Mouse PRDC/GREM2 Protein
Brand: R&D Systems
SKU: 2069-PR
Price: 394 USD

Catalog # 2069-PR | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

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Key Product Details

Source:	E. coli
Accession #:	NP_035955
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse PRDC/GREM2 Protein

Source	E. coli-derived mouse PRDC/GREM2 protein Arg22-Gln168, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17.1 kDa (monomer)
Activity	Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.03-0.12 µg/mL in the presence of 50 ng/mL of rhBMP-4.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

2069-PR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2069-PR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRDC/GREM2

PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond (1, 2). Some members of this family, including PRDC, have an additional cysteine residue used for dimerization (1, 2). Of all the CAN family members, PRDC is most closely related to Gremlin, displaying 52% amino acid sequence identity. PRDC was first identified in a screen for developmentally regulated genes by gene trapping in embryonic stem cells (3). PRDC expression is detected by in situ hybridization in the dorsal edge of the spinal cord at E10.5, in commissural neurons in the caudal part of the spinal cord two days later (3), and in the granulosa cells of selective ovarian follicles (4). In the adult, abundant levels of PRDC are detected by RT-PCR in the mouse ovary, brain, and spleen, and to a lesser degree in the colon, kidney, lung, liver, and uterus (4). PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta (4). Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone (4).

Item	Value
References	Pearce, J. et al. (1999) Dev. Biol. 209:99. Avsian-Kretchmer, O. and A. Hsueh (2004) Molecular Endocrinology 18:1. Minabe-Saegusa, C. et al. (1998) Develop. Growth Differ. 40:343. Sudo, S. et al. (2004) Jour. Biol. Chem. 279:23134.
Long Name	Protein Related to DAN and Cerberus/Gremlin-2
Entrez Gene IDs	64388 (Human); 23893 (Mouse)
Alternate Names	CKTSF1B2, CKTSF1B2FLJ21195, Cysteine knot superfamily 1, BMP antagonist 2, DAN domain family member 3, DAND3, DAND3Prdc, GREM2, PRDC, PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis), Protein related to DAN and cerberus, gremlin 2, gremlin 2, cysteine knot superfamily, homolog, gremlin-2

Citations for Recombinant Mouse PRDC/GREM2 Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PRDC/GREM2 Protein include:

Species: Mouse
Sample Types: Whole Cell
Applications: Bioassay

F Gao et al. (2022-04-05), Hedgehog-responsive PDGFRa(+) fibroblasts maintain a unique pool of alveolar epithelial progenitor cells during alveologenesis Cell Reports, 2022-039(1):110608.
PMID: 35385750
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

T He et al. (2022-03-06), Compensatory roles of Protein Related to DAN and Cerberus (PRDC) decrease in pulmonary arterial hypertension International journal of biological sciences, 2022-018(6):2372-2391.
PMID: 35414785
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

M Cassandras et al. (2020-10-12), Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung Nat Cell Biol, 2020-10(0).
PMID: 33046884
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Tanwar V et al. (2014-07-01), Gremlin 2 promotes differentiation of embryonic stem cells to atrial fate by activation of the JNK signaling pathway. Stem Cells, 2014-032(7):1774-88.
PMID: 24648383

Recombinant Mouse PRDC/GREM2 Protein

Catalog # 2069-PR | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse PRDC/GREM2 Protein

Formulation, Preparation and Storage

Carrier Free

2069-PR

Carrier

2069-PR/CF

Carrier Free

Reconstitution Calculator

Background: PRDC/GREM2

Citations for Recombinant Mouse PRDC/GREM2 Protein (4)

Product Documents for Recombinant Mouse PRDC/GREM2 Protein

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