Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Catalog # 10094-PS | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q01102

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse P-Selectin/CD62P protein
Trp42-Ala709, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp42

Predicted Molecular Mass

73.4 kDa

SDS-PAGE

105-125 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Recombinant Mouse P-Selectin/CD62P His-tag Protein Bioactivity

Recombinant Mouse P-Selectin/CD62P His-tag Protein Bioactivity

Immobilized Recombinant Mouse P‑Selectin/CD62P His-tag (Catalog # 10094-PS) supports the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.15‑0.9 μg/mL.
Recombinant Mouse P-Selectin/CD62P His-tag Protein SDS-PAGE

Recombinant Mouse P-Selectin/CD62P His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse P‑Selectin/CD62P His-tag (Catalog # 10094-PS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa.

Formulation, Preparation and Storage

10094-PS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a cell surface glycoprotein of the Selectin family and is expressed by activated platelets and endothelial cells (1-3). Mouse P-Selectin cDNA encodes a 768 amino acid (aa) type I transmembrane protein with a signal peptide, a large extracellular domain (ECD), a transmembrane domain and a short cytoplasmic domain (4). The mature ECD of mouse P-Selectin contains a C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains (5). The mature ECD of mouse P-selectin shares 66% and 89% aa sequence identity with human and rat, respectively. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides (6). P-Selectin binds to P-Selectin glycoprotein ligand-1 (PSGL-1), a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes (7). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium (8). This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (1, 3).

References

  1. Kansas, G.S. (1996) Blood 88:3259.
  2. Lü, S. (2015) PLOS One 10:1.
  3. Edwards, E.E. (2017) Integr Biol (Camb) 9:313.
  4. Patel, K.D. (1995) J. Cell Biol. 131:1893.
  5. McEver, R.P. (1997) J. Clin. Invest. 100:485.
  6. Zhang, N. (2016) Arterioscler Thromb Vasc Biol 36:1114.
  7. Luo, W. (2012) Atherosclerosis 220:110.
  8. Zuchtriegel, G. (2016) PLOS Biology 14:1.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human); 20344 (Mouse)

Gene Symbol

SELP

UniProt

Q01102

Product Documents for Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

For research use only

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