Recombinant Mouse LRP-6 His Tagged Protein, CF

Name: Recombinant Mouse LRP-6 His Tagged Protein, CF
Brand: R&D Systems
SKU: 2960-LR
Rating: 4 (2 reviews)

Catalog # 2960-LR | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	O88572
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse LRP-6 His Tagged Protein, CF

Product Specifications for Recombinant Mouse LRP-6 His Tagged Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse LRP-6 protein Ala20-Pro1368, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala20
Predicted Molecular Mass	152.6 kDa
SDS-PAGE	170 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. rmLRP-6 binds rhDkk-1, biotin with an apparent K_D <15 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rmLRP-6 with a linear range of 0.2-10 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2960-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

Item	Value
References	Schneider, W.J. and J. Nimpf (2003) Cell Mol Life Sci. 60:892. He, X. et al. (2004) Development 131:1663. Seto, E.S. and H.J. Bellen (2004) Trends Cell Biol. 14:45. Pinson, K.I. et al. (2000) Nature 407:535. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879. Tamai, K. et al. (2000) Nature 407:530. Tamai, K. et al. (2004) Mol. Cell 13:149. Liu, G. et al. (2003) Mol. Cell. Biol. 23:5825. Mao, B. et al. (2001) Nature 411:321. Semenov, M.V. et al. (2001) Curr. Biol. 11:951. Bafico, A. et al. (2001) Nat. Cell Biol. 3:683. Li, X. et al. (2005) J. Biol. Chem. 280:19883. Semenov, M. et al. (2005) J. Biol. Chem. 280:26770. Itasaki, N. et al. (2003) Development 130:4295. Kelly, O. et al. (2004) Development 131:2803.
Long Name	LDL Receptor-related Protein 6
Entrez Gene IDs	4040 (Human); 16974 (Mouse)
Alternate Names	ADCAD2, FLJ90062, FLJ90421, LRP-6, LRP6, low density lipoprotein receptor-related protein 6, low-density lipoprotein receptor-related protein 6

Citations for Recombinant Mouse LRP-6 His Tagged Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse LRP-6 His Tagged Protein, CF include:

Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Capture

R Chidiac et al. (2021-06-09), A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy Embo Molecular Medicine, 2021-00(0):e13977.
PMID: 34105895
Species: Mouse
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Liu S et al. (2013-10-23), CCN2 modulates hair follicle cycling in mice. Mol Biol Cell, 2013-124(24):3939-44.
PMID: 24152728
Species: Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Krause C et al. (2010-10-15), Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways. J. Biol. Chem., 2010-1285(53):41614-26.
PMID: 20952383

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Customer Reviews for Recombinant Mouse LRP-6 His Tagged Protein, CF (2)

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Recombinant Mouse LRP-6 His Tagged Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 08/19/2023
Recombinant Mouse LRP-6 His Tagged Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 08/19/2023

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Recombinant Mouse LRP-6 His Tagged Protein, CF

Catalog # 2960-LR | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse LRP-6 His Tagged Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LRP-6

Citations for Recombinant Mouse LRP-6 His Tagged Protein, CF (3)

Customer Reviews for Recombinant Mouse LRP-6 His Tagged Protein, CF (2)

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Recombinant Mouse LRP-6 His Tagged Protein, CF

Recombinant Mouse LRP-6 His Tagged Protein, CF

Product Documents for Recombinant Mouse LRP-6 His Tagged Protein, CF

Product Datasheets

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