Recombinant Mouse JAM-C Fc Chimera Protein, CF

Name: Recombinant Mouse JAM-C Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1213-J3

Catalog # 1213-J3 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q9D8B7
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse JAM-C Fc Chimera Protein, CF

Technical Data
Citations (5) / Reviews

Product Specifications for Recombinant Mouse JAM-C Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse JAM-C protein

Mouse JAM-C (Val32 - Asn241) Accession # Q9D8B7	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val32

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

50 kDa (monomer)

Activity

Measured by its ability to inhibit the adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes on immobilized JAM-2 (VE-JAM)/Fc Chimera. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 1 x 10⁵ cells/well. Each well was coated with 100 µL of a 0.2 µg/mL solution of rhJAM-2 (VE-JAM)/Fc Chimera.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1213-J3

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JAM-C

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

Item	Value
References	Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. Aurrand-Lions, M. et al. (2001) Blood 98:3699. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733. Johnson-Leger, C. et al. (2002) Blood 100:25793.
Long Name	Junctional Adhesion Molecule C
Entrez Gene IDs	83700 (Human); 83964 (Mouse)
Alternate Names	CD323, JAM-2, JAM-C, JAM-CFLJ14529, JAM3, JAMC, junctional adhesion molecule 3JAM-3, junctional adhesion molecule C

Citations for Recombinant Mouse JAM-C Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse JAM-C Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture

M Czabanka et al. (2020-02-11), Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis Int J Mol Sci, 2020-021(4).
PMID: 32054130
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

V Radulovic et al. (2019-06-04), Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis Cell Rep, 2019-027(10):2826-2836.e5.
PMID: 31167130
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

E Hintermann et al. (2016-04-25), Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016-010(4):419-33.
PMID: 27111582
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Su RW et al. (2012-04-12), Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF. PLoS ONE, 2012-07(4):e34325.
PMID: 22511936
Species: Mouse
Sample Types: Whole Cells
Applications: Adhesion Assay, Flow Cytometry

Arcangeli ML et al. (2011-08-25), JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood, 2011-0118(17):4609-19.
PMID: 21868569

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Showing 1 - 5 of 5 citations Showing All

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