Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Name: Recombinant Mouse IL-1 alpha/IL-1F1 Protein
Brand: R&D Systems
SKU: 400-ML
Rating: 5 (6 reviews)

Catalog # 400-ML | R&D Systems, Inc. a Bio-Techne Brand

(6)

Citations (22)

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Key Product Details

Source:	E. coli
Accession #:	Q62161
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Product Specifications for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Source	E. coli-derived mouse IL-1 alpha/IL-1F1 protein Ser6-Ser161
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser6
Predicted Molecular Mass	18 kDa
Activity	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 3-7 pg/mL.

Scientific Data Examples for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

	Recombinant Mouse IL-1 alpha/IL-1F1 Protein Bioactivity Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED₅₀ for this effect is 3-7 pg/mL.
	Recombinant Mouse IL-1 alpha/IL-1F1 Protein SDS-PAGE 1 μg/lane of Recombinant Mouse IL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

400-ML

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

400-ML/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Item	Value
Long Name	Interleukin 1 alpha
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names	BAF, Hematopoietin-1, IL-1 alpha, IL-1A, IL-1F1, IL1, IL1 alpha, IL1-ALPHA, IL1A, IL1F1, IL1F1hematopoietin-1, LAF, LEM, interleukin 1, alpha, interleukin-1 alpha, preinterleukin 1 alpha, pro-interleukin-1-alpha

Citations for Recombinant Mouse IL-1 alpha/IL-1F1 Protein (22)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-1 alpha/IL-1F1 Protein include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Matsunami et al. (2021-06-18), Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells Journal of oral biosciences, 2021-00(0).
PMID: 34153475
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

KE Salmeron et al. (2019-11-14), Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke J Neuroinflammation, 2019-116(1):222.
PMID: 31727174
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

N Ketelut-Ca et al. (2019-08-19), Caspase-11-dependent IL-1Î± release boosts Th17 immunity against Paracoccidioides brasiliensis PLoS Pathog., 2019-015(8):e1007990.
PMID: 31425553
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

H Lindahl et al. (2019-07-10), IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019-00(0).
PMID: 31292217
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Liu et al. (2018-09-07), Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation Cell. Physiol. Biochem., 2018-049(3):1180-1196.
PMID: 30196277
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

PC Duncker et al. (2017-12-29), GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction J. Immunol., 2017-10(0).
PMID: 29288202
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jiang H et al. (2015-12-23), LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production. J Immunol, 2015-1196(3):1081-90.
PMID: 26700766
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jules J et al. (2015-08-14), The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis. J Biol Chem, 2015-0290(39):23738-50.
PMID: 26276390
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lin D et al. (2015-04-29), Secreted IL-1alpha promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice. Eur J Immunol, 2015-045(7):2084-98.
PMID: 25870999
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Rabeony H et al. (2014-07-10), Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 2014-09(7):e101937.
PMID: 25010647
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chen D et al. (2014-06-30), Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. Cancer Res, 2014-074(16):4270-81.
PMID: 24980554
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Szewczyk K et al. (2013-03-21), Distinctive subdomains in the resorbing surface of osteoclasts. PLoS ONE, 2013-08(3):e60285.
PMID: 23555944
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jayaraman P et al. (2013-03-13), IL-1beta promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol, 2013-0190(8):4196-204.
PMID: 23487424
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2012-07-16), Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-0209(8):1505-17.
PMID: 22802353
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kayamuro H et al. (2010-09-29), Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-084(24):12703-12.
PMID: 20881038
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Guilloteau K et al. (2010-03-24), Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010-0184(0):5263-70.
PMID: 20335534
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dienz O et al. (2009-01-12), The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells. J. Exp. Med., 2009-0206(1):69-78.
PMID: 19139170
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chackerian AA et al. (2007-08-15), IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol., 2007-0179(4):2551-5.
PMID: 17675517
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Li W et al. (2007-03-30), Protection of CD8+ T cells from activation-induced cell death by IL-18. J. Leukoc. Biol., 2007-082(1):142-51.
PMID: 17400610
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wei S et al. (2005-02-01), IL-1 mediates TNF-induced osteoclastogenesis. J. Clin. Invest., 2005-0115(2):282-90.
PMID: 15668736
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Scharstuhl A et al. (2002-12-01), Loss of transforming growth factor counteraction on interleukin 1 mediated effects in cartilage of old mice. Ann. Rheum. Dis., 2002-161(12):1095-8.
PMID: 12429542
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Coope HJ et al. (2002-10-15), CD40 regulates the processing of NF-kappaB2 p100 to p52. EMBO J., 2002-121(20):5375-85.
PMID: 12374738

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/07/2021
Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 03/06/2021
Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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Application: Stem/Immune cell maintenance or differentiation

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Application: In vitro bioactivity in cell culture

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Application: Cell migration/motility

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Catalog # 400-ML | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Scientific Data Examples for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Formulation, Preparation and Storage

Carrier Free

400-ML

Carrier

400-ML/CF

Carrier Free

Reconstitution Calculator

Background: IL-1 alpha/IL-1F1

Citations for Recombinant Mouse IL-1 alpha/IL-1F1 Protein (22)

Customer Reviews for Recombinant Mouse IL-1 alpha/IL-1F1 Protein (6)

Customer Images

Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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