Recombinant Mouse IFN-beta (Mammalian) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 12410-1
A New rm IFN-beta is Available! It has lower endotoxin specifications, is made in-house, and is priced competitively!
Key Product Details
- R&D Systems N/A-derived Recombinant Mouse IFN-beta (Mammalian) Protein (12410-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Gene was obtained from mouse DNA expressed in mammalian cells.
Accession # K00020
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
19.6 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as listed
Mouse (L929/EMCV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~2.5 U/ml. Lot Activity was derived from multiple determinations in the above assay. The units are determined by use of a Mu-IFN-beta laboratory standard calibrated to the international reference standard for mouse interferon beta provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1- 2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Formulation, Preparation and Storage
12410-1
| Formulation | 20 mM Sodium Phosphate, pH 6.4; 300 mM NaCl; 5% Glycerol |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IFN-beta
IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
UniProt
Additional IFN-beta Products
Product Documents for Recombinant Mouse IFN-beta (Mammalian) Protein, CF
Product Specific Notices for Recombinant Mouse IFN-beta (Mammalian) Protein, CF
For research use only
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