Recombinant Mouse Galectin-1 Protein

Name: Recombinant Mouse Galectin-1 Protein
Brand: R&D Systems
SKU: 1245-GA

Catalog # 1245-GA | R&D Systems, Inc. a Bio-Techne Brand

Citations (12)

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Key Product Details

Source:	E. coli
Accession #:	P16045
Applications:	Bioactivity

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Technical Data
Citations (12) / Reviews

Product Specifications for Recombinant Mouse Galectin-1 Protein

Source	E. coli-derived mouse Galectin-1 protein Ala2-Glu135
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala2
Predicted Molecular Mass	15 kDa
SDS-PAGE	15 kDa
Activity	Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀ for this effect is 1-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1245-GA

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1245-GA/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-1

Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family (1-3). Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways (1). Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation (1, 2, 4-6). Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha ₄ beta ₁, alpha ₅ beta ₁ and alpha ₄ beta ₇, and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells (2, 3, 6-11). Most of this expression is cytosolic. Mouse Galectin-1 shares 88% aa sequence identity with human, 96% with rat, and 84% with equine, ovine, bovine and porcine Galectin-1. Endothelial cell surface expression, including tumor endothelial cells, is greatly increased by cell activation (9). Galectin-1 is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege (5, 12). Its immunosuppressive properties appear to also allow tumor cells to evade immune detection (4, 5). It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin‑1‑binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized (4, 13). It also induces apoptosis of immature thymocytes (3, 6). Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling (3). The dimer form of Galectin-1 also induces neutrophil down‑regulation by inducing cell surface exposure of phosphatidylserine that marks the cell for phagocytosis (14). Galectin-1 can also modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage (1, 2).

Item	Value
References	Di Lella, S. et al. (2011) Biochemistry 50:7842. Rabinovich, G.A. and J.M. Ilarregui (2009) Immunol. Rev. 230:144. Rabinovich, G.A. and M. Vidal. (2011) Curr. Opin. Hematol. 18:443. Cedeno-Laurent, F. and C.J. Dimitroff (2012) Clin. Immunol. 142:107. Than, N.G. et al. (2012) Trends Endocrinol. Metab. 23:23. Rubinstein, N. et al. (2004) Tissue Antigens 64:1. Courad, P.O. et al. (1989) J. Biol. Chem. 264:1310. Hirabayashi, J. et al. (1989) Biochim. Biophys. Acta 1008:85. Thijssen, V.L. et al. (2008) Am. J. Pathol. 172:545. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41. Dias-Baruffi, M. et al. (2010) Glycobiology 20:507. Than, N.G. et al. (2008) Proc. Natl. Acad. Sci. USA 105:15819. Toscano, M.A. et al. (2007) Nat. Immunol. 8:825. Stowell, S.R. et al. (2009) J. Biol. Chem. 284:4989.
Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Alternate Names	14 kDa laminin-binding protein, 14 kDa lectin, BHL, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GAL1, GBP, Galaptin, Galectin-1, Galectin1, HBL, HLBP14, HPL, L-14, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, gal-1, galectin 1, lectin, galactoside-binding, soluble, 1

Citations for Recombinant Mouse Galectin-1 Protein (12)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Galectin-1 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

W Han et al. (2023-03-01), Dying transplanted neural stem cells mediate survival bystander effects in the injured brain Cell Death Disease, 2023-03-01;14(3):173.
PMID: 36854658
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Standard

LG Morosi et al. (2021-06-18), Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits Science Advances, 2021-07(25).
PMID: 34144987
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JY Ge et al. (2020-09-29), Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 2020-011(1):425.
PMID: 32993816
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JM Lykken et al. (2016-02-17), Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms Blood, 2016-0127(15):1886-95.
PMID: 26888257
Species: Mouse
Sample Types: Protein
Applications: Bioassay

Madireddi S et al. (2014-06-23), Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med, 2014-0211(7):1433-48.
PMID: 24958847
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lim M et al. (2014-06-11), Induction of galectin-1 by TGF-beta1 accelerates fibrosis through enhancing nuclear retention of Smad2. Exp Cell Res, 2014-0326(1):125-35.
PMID: 24928277
Species: Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

Qu WS et al. (2010-12-02), Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. J. Neurochem., 2010-1116(2):217-26.
PMID: 21054390
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Cedeno-Laurent F et al. (2010-09-15), Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation. J. Immunol., 2010-0185(8):4659-72.
PMID: 20844192
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Tsai CM et al. (2008-10-01), Galectin-1 promotes immunoglobulin production during plasma cell differentiation. J. Immunol., 2008-1181(7):4570-9.
PMID: 18802059
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Plachta N et al. (2007-05-07), Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci., 2007-010(6):712-9.
PMID: 17486104
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Silva-Monteiro E et al. (2007-02-01), Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection. Am. J. Pathol., 2007-0170(2):546-56.
PMID: 17255323
Species: Mouse
Sample Types: N/A, Whole Cells
Applications: Bioassay, Western Blot

Endharti AT et al. (2005-01-01), Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur. J. Immunol., 2005-035(1):86-97.
PMID: 15593120

Recombinant Mouse Galectin-1 Protein

Catalog # 1245-GA | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Mouse Galectin-1 Protein

Formulation, Preparation and Storage

Carrier Free

1245-GA

Carrier

1245-GA/CF

Carrier Free

Reconstitution Calculator

Background: Galectin-1

Citations for Recombinant Mouse Galectin-1 Protein (12)

Product Documents for Recombinant Mouse Galectin-1 Protein

Product Datasheets

COA

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