Recombinant Mouse EGFR Fc Chimera Protein, CF

Name: Recombinant Mouse EGFR Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1280-ER
Rating: 4 (1 reviews)

Catalog # 1280-ER | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9EP98
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse EGFR Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse EGFR Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse EGFR protein

Mouse EGFR (Leu25-Ser647) Accession # Q9EP98	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu25

Predicted Molecular Mass

95.9 kDa (monomer)

SDS-PAGE

135-150 kDa, reducing conditions

Activity

Measured by its ability to bind recombinant human EGF in a functional ELISA with an estimated K_d of <10 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1280-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44%-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

Item	Value
References	Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183. Shilo, B.Z. (2005) Development 132:4017. Avivi, A. et al. (1991) Oncogene 6:673. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050. Reiter J.L. et al. (2001) Genomics 71:1. Xu, Y.H. et al. (1984) Nature 309:806. Graus-Porta, D. et al. (1997) EMBO J. 16:1647. Yarden, Y. et al. (1987) Biochemistry 26:1434. Burgess, A.W. et al. (2003) Mol. Cell 12:541. Lemmon, M.A. et al. (1997) EMBO J. 16:281. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523. Sibilia, M. and E.F. Wagner (1995) Science 269:234. Miettinen, P.J. et al. (1995) Nature 376:337. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.
Long Name	Epidermal Growth Factor Receptor
Entrez Gene IDs	1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EGF R, EGFR, ERBB1PIG61, ErbB, ErbB1, HER-1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), mENA

Citations for Recombinant Mouse EGFR Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse EGFR Fc Chimera Protein, CF include:

Species: Human
Sample Types: RNA
Applications: Binding Assay

Li N et al. (2011-06-08), Inhibition of Cell Proliferation by an Anti-EGFR Aptamer. PLoS ONE, 2011-06(6):e20299.
PMID: 21687663

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Recombinant Mouse EGF R/ErbB1 Fc Chimera Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Reason for Rating: background is low, signal is good

Verified Customer | Posted 05/18/2016

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Product Documents for Recombinant Mouse EGFR Fc Chimera Protein, CF

Product Datasheets

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Recombinant Mouse EGFR Fc Chimera Protein, CF

Catalog # 1280-ER | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Mouse EGFR Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: EGFR

Citations for Recombinant Mouse EGFR Fc Chimera Protein, CF (1)

Customer Reviews for Recombinant Mouse EGFR Fc Chimera Protein, CF (1)

Recombinant Mouse EGF R/ErbB1 Fc Chimera Protein, CF

Product Documents for Recombinant Mouse EGFR Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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