Recombinant Mouse Agrin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11573-AG

R&D Systems, part of Bio-Techne
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11573-AG-100
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Agrin His-tag Protein (11573-AG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession #

A2ASQ1.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Agrin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Agrin protein
Ala987-Leu1950 with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His (of 6-His tag)

Predicted Molecular Mass

105 kDa

SDS-PAGE

114-128 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse Agrin His-tag binds to Recombinant Mouse LRP-4 His-tag Protein (Catalog # 10229-LR) with an ED50 of 30.0-300 ng/mL.

Scientific Data Images for Recombinant Mouse Agrin His-tag Protein, CF

Recombinant Mouse Agrin His-tag Protein SEC-MALS.

Recombinant Mouse Agrin His-tag (Catalog # 11573-AG) has a molecular weight (MW) of 120.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Mouse Agrin His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Mouse Agrin His-tag Protein (Catalog # 11573-AG) binds to Recombinant Mouse LRP-4 His-tag Protein (10229-LR) with an ED50 of 30.0-300 ng/mL.

Recombinant Mouse Agrin His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse Agrin His-tag Protein (Catalog # 11573-AG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-128 kDa under reducing conditions.

Formulation, Preparation and Storage

11573-AG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Agrin

Agrin is a 400‑600 kDa heparan sulfate proteoglycan component of the extracellular matrix. The N‑terminal half of mouse Agrin, which mediates ECM interactions, contains nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C-terminal half contains four EGF-like repeats and three Laminin globular G domains. Human Agrin also contains a Laminin‑binding N-terminal Agrin domain (NtA), and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C-terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1-3). This recombinant protein consists of the C-terminal half of mouse Agrin. It shares 59%, 82%, and 94% aa sequence identity with comparable regions of chick, human, and rat Agrin, respectively. The C-terminal half of Z- and Z+ Agrin binds to alpha-Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4-6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin‑induced AChR clustering requires a myotube receptor complex that contains alpha-Dystroglycan, MuSK, and LRP4 (4, 9-11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer’s disease plaques where it binds the A beta (1-40) peptide and promotes amyloid fibril formation (12). It regulates neuronal excitability by binding and inhibiting the alpha3 subunit of the neuronal Na/K ATPase (13). It functions as an epithelial cell attachment receptor for HIV-1 through interactions with the gp41 coat protein (14). During T cell activation, Agrin contributes to formation of the immunological synapse and regulates the threshold of T cell activation (15).

References

  1. Jury, E.C. and P.S. Kabouridis (2010) Arthritis Res. Ther. 12:205.
  2. Bezakova, G. and M.A. Ruegg (2003) Nat. Rev. Mol. Cell Biol. 4:295.
  3. Rupp, F. et al. (1991) Neuron 6:811.
  4. Gee, S.H. et al. (1994) Cell 77:675.
  5. Sugiyama, J. et al. (1994) Neuron 13:103.
  6. Gesemann, M. et al. (1998) J. Biol. Chem. 273:600.
  7. Burgess, R.W. et al. (1999) Neuron 23:33.
  8. Ferns, M.J. et al. (1993) Neuron 11:491.
  9. Glass, D.J. et al. (1996) Cell 85:513.
  10. Kim, N. et al. (2008) Cell 135:334.
  11. Zhang, B. et al. (2008) Neuron 60:285.
  12. Cotman, S.L. et al. (2000) Mol. Cell. Neurosci. 15:183.
  13. Hilgenberg, L.G.W. et al. (2006) Cell 125:359.
  14. Alfsen, A. et al. (2005) Mol. Biol. Cell 16:4267.
  15. Khan, A.A. et al. (2001) Science 292:1681.

Alternate Names

AGRN

Entrez Gene IDs

375790 (Human); 11603 (Mouse); 25592 (Rat)

Gene Symbol

AGRN

UniProt

A2ASQ1.1

Additional Agrin Products

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Product Specific Notices for Recombinant Mouse Agrin His-tag Protein, CF

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