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Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1477-AR

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1477-AR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Activin RIB/ALK-4 protein
Mouse Activin RIB
(Leu32 - Glu126)
Accession #NP_031421
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

N-terminal Sequence Analysis

Leu32

Predicted Molecular Mass

37.2 kDa (monomer)

SDS-PAGE

44 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmActivin RIB/Fc Chimera at 1 µg/mL (100 µL/well) can bind rmCripto with a linear range of 0.8‑100 ng/mL.

Formulation, Preparation and Storage

1477-AR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIB/ALK-4

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23% - 38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5 - 7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

References

  1. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
  3. Tsuchida, K. et al. (1995) Endocrinology 136:5493.
  4. Ebner, R. et al. (1993) Science 260:1344.
  5. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
  6. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Gu, Z. et al. (1998) Genes Dev. 12:844.
  10. Chen, Y. et al. (2004) Dev. Biol. 268:280.

Long Name

Activin Receptor IB

Alternate Names

ActivinRIB, ACVR1B, ALK-4, ALK4

Entrez Gene IDs

91 (Human); 11479 (Mouse)

Gene Symbol

ACVR1B

UniProt

Additional Activin RIB/ALK-4 Products

Product Documents for Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

For research use only

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Reconstitution Calculator

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