Recombinant Human TFPI Protein, CF

Name: Recombinant Human TFPI Protein, CF
Brand: R&D Systems
SKU: 2974-PI

Catalog # 2974-PI | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	P10646
Applications:	Inhibition Activity

View Full Technical Details for Recombinant Human TFPI Protein, CF

Technical Data
Citations (4) / Reviews

Product Specifications for Recombinant Human TFPI Protein, CF

Source	Mouse myeloma cell line, NS0-derived human TFPI protein Asp29-Lys282, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp29
Predicted Molecular Mass	31 kDa
SDS-PAGE	31 kDa
Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog # ES002). The IC₅₀ value is <0.75 nM, as measured under the described conditions.

Scientific Data Examples for Recombinant Human TFPI Protein, CF

	Recombinant Human TFPI Protein Enzyme Activity Recombinant Human TFPI (Catalog # 2974-PI) inhibits trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2974-PI

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified Recombinant Human TFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).

Item	Value
References	Bai, H. et al. (2005) Thromb Haemost. 93:1055. Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959. Mine, S. et al. (2002) Biochemistry 41:78.
Long Name	Tissue Factor Pathway Inhibitor
Entrez Gene IDs	7035 (Human); 21788 (Mouse)
Alternate Names	EPI, EPITFPI1LACITFI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor, TFPI, convertin, tissue factor pathway inhibitor, tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

Citations for Recombinant Human TFPI Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TFPI Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Bhargavan et al. (2022-09-09), SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis International Journal of Molecular Sciences, 2022-023(18).
PMID: 36142345
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

LT Hsieh et al. (2022-01-31), Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022-018(1):e1010280.
PMID: 35100311
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Che S et al. (2015-04-07), Tissue factor-expressing tumor cells can bind to immobilized recombinant tissue factor pathway inhibitor under static and shear conditions in vitro. PLoS ONE, 2015-010(4):e0123717.
PMID: 25849335
Sample Types: N/A

Nicol GR et al. (2008-04-03), Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients. Mol. Cell Proteomics, 2008-07(10):1974-82.
PMID: 18388126

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Showing 1 - 4 of 4 citations Showing All

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