Recombinant Human Ret His-tag Protein

R&D Systems, part of Bio-Techne | Catalog # 1168-CR

R&D Systems, part of Bio-Techne
Discontinued Product
1168-CR has been discontinued. An alternative/replacement product is available: 10302-CR. View all Ret products.

Key Product Details

Source

Sf 21 (stably transfected)

Accession #

P07949

Conjugate

Unconjugated

Applications

Binding Activity

View all Ret Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Ret protein
Leu29-Arg635, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

69 kDa

SDS-PAGE

90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Ret His-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Scientific Data Images for Recombinant Human Ret His-tag Protein

Recombinant Human Ret Protein Binding Activity

Recombinant Human Ret Protein Binding Activity

When Recombinant Human Ret His-tag (Catalog # 1168-CR) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Formulation, Preparation and Storage

1168-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha-1 - 4). GFR alpha-1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1 - 4).

References

  1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  4. Baloh, R. et al. (1998) Neuron 21:1291.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

P07949

Additional Ret Products

Product Documents for Recombinant Human Ret His-tag Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ret His-tag Protein

For research use only

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