Recombinant Human p53 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # SP-454

R&D Systems, part of Bio-Techne
Discontinued Product
SP-454 has been discontinued. View all p53 products.

Key Product Details

Source

E. coli

Accession #

P04637

Conjugate

Unconjugated

Applications

Bioactivity

View all p53 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human p53 protein

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

44 kDa

Activity

Recombinant Human p53 is ideal for use as a control substrate for in vitro Ubiquitin conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human p53 concentration of 0.5-2.5 μM.

Formulation, Preparation and Storage

SP-454
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30 µM ZnCl2
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: p53

p53 is well known for its key role as a tumor suppressor protein.  It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range of factors that promote DNA repair mechanisms or apoptosis, including  cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).

References

  1. Arrowsmith, C.H. et al. (1999) Cell Death Differ. 6:1169.
  2. Dötsch, V. et al. (2010) Cold Spring Harb. Perspect. Biol. 2:a004887.
  3. Freed-Pastor, W.A. & C. Prives (2012) Genes Dev. 26:1268.
  4. Feng, Z. et al. (2011) J. Mol. Cell Biol. 3:44.
  5. Gu, B. & W.-G. Zhu (2012) Int. J. Biol. Sci. 8:672.
  6. Momand, J. et al. (1992) Cell 69:1237.
  7. Haupt, Y. et al. (1997) Nature 387:296.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for Recombinant Human p53 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human p53 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.