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Recombinant Human N-Acetylglucosaminyltransferase1/MGAT1, CF

Catalog # 8334-GT | R&D Systems, Inc. a Bio-Techne Brand
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8334-GT-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human N-Acetylglucosaminyltransferase I/MGAT1 protein
Thr30-Asn445, with C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

N-terminal Sequence Analysis

Thr30

Predicted Molecular Mass

48 kDa

SDS-PAGE

45-52 kDa, reducing conditions

Activity

Measured by its ability to transfer N-Acetyl-D-Glucosamine from UDP-GlcNAc to alpha1-3, alpha1-6-Mannotriose.
The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8334-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: N-Acetylglucosaminyltransferase I/MGAT1

Mannosylglycoprotein N-acetyl-glucosaminyltransferase 1 (MGAT1), also known as GnT I, is a type II transmembrane Golgi enzyme that regulates the branching of N‑glycans. By transferring a GlcNAc residue to the alpha3-linked mannose of the trimannosyl core of N-linked oligosaccharides, MGAT1 initiates the formation of complex and hybrid N-linked carbohydrates (1). Mice lacking MGAT1 activity die at mid-gestation, revealing an essential role for these carbohydrates (2). Branched N‑glycans on cell surface proteins bind to galectins and allow the formation of a multivalent lattice thereby enhancing cell surface residency of growth factor receptors and focal adhesion proteins. Because of its key role in N-glycan synthesis, MGAT1 is a potential target for anti-cancer therapy (3). Enzymatic activity of the recombinant human MGAT1 was determined using a phosphatase coupled glycosyltransferase assay (4).

References

  1.  Kumar R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9948.
  2.  Ioffe, E. and Stanley, P.  (1994) Proc. Natl. Acad. Sci. USA 91:728.
  3. Zavareh, R. et al. (2012) PLoS ONE 7:e43721.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

Alternate Names

GGNT1, GlcNac-TI, GLCT1, GNT-1

Entrez Gene IDs

4245 (Human); 17308 (Mouse); 81519 (Rat)

Gene Symbol

MGAT1

UniProt

Additional N-Acetylglucosaminyltransferase I/MGAT1 Products

Product Documents for Recombinant Human N-Acetylglucosaminyltransferase1/MGAT1, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human N-Acetylglucosaminyltransferase1/MGAT1, CF

For research use only

Citations for Recombinant Human N-Acetylglucosaminyltransferase1/MGAT1, CF (3)

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