Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

Name: Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1599-MB
Price: 394 USD

Catalog # 1599-MB | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_005922
Applications:	Binding Activity

View Full Technical Details for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

Product Specifications for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human MICB protein

Human MICB (Ala23-Gly298) (Asn74Asp) & (Glu24-Gly298) (Asn74Asp) Accession # NP_005922	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala23 & Glu24

Predicted Molecular Mass

58 kDa

SDS-PAGE

82 kDa, reducing conditions

Activity

Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1599-MB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

Item	Value
References	Groh, V. et al. (2001) Nature Immunol. 2:255. Stephens, H. (2001) Trends Immunol. 22:378. Bauer, S. et al. (1999) Science 285:727. Groh, V. et al. (2002) Nature 419:734. Steinle, A. et al. (2001) Immunogenetics 53:279. Pende, D. et al. (2002) Cancer Res. 62:6178. Salih, H. et al. (2003) Blood 102:1389.
Long Name	MHC Class I-related Protein B
Entrez Gene IDs	4277 (Human)
Alternate Names	MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, MICB, PERB11.2MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog

Citations for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JA Myers et al. (2022-08-08), Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion JCI Insight, 2022-00(0).
PMID: 35727627
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

AB Raneros et al. (2017-05-09), Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017-08(19):31959-31976.
PMID: 28404876
Applications: Bioassay

S Weil et al. (2017-04-10), Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma Front Immunol, 2017-08(0):387.
PMID: 28443091
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kwong KY et al. (2008-09-16), Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J. Mol. Biol., 2008-0384(5):1143-56.
PMID: 18809410
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Spreu J et al. (2006-09-01), Human cytomegalovirus-encoded UL16 discriminates MIC molecules by their alpha2 domains. J. Immunol., 2006-0177(5):3143-9.
PMID: 16920952

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Showing 1 - 5 of 5 citations Showing All

Product Documents for Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

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Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF

Catalog # 1599-MB | R&D Systems, Inc. a Bio-Techne Brand

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