Recombinant Human KGF/FGF-7 Protein, Animal-Free Best Seller

Name: Recombinant Human KGF/FGF-7 Protein, Animal-Free
Brand: R&D Systems
SKU: BT-KGF-AFL

R&D Systems, part of Bio-Techne | Catalog # BT-KGF-AFL

Submit a review

Product Datasheet / COA / SDS

Key Product Details

Learn more about Animal-Free Recombinant Proteins

Source:	E. coli
Accession #:	P21781.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human KGF/FGF-7 Protein, Animal-Free

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human KGF/FGF-7 Protein, Animal-Free

Source

E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys32 & Asn33

Predicted Molecular Mass

19 kDa

SDS-PAGE

19 kDa

Activity

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.

The specific activity of Animal-Free™ Recombinant Human KGF/FGF‑7 is >8.16 x 10⁵ units/mg, which is calibrated against the human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).

Scientific Data Examples for Recombinant Human KGF/FGF-7 Protein, Animal-Free

	Equivalent Bioactivity of GMP, Animal-Free and RUO grades of Recombinant Human KGF/FGF-7 Protein. Equivalent bioactivity of GMP (BT-KGF-GMP), Animal-Free (Catalog # BT-KGF-AFL) and RUO (BT-KGF) grades of Recombinant Human KGF/FGF-7 GMP protein as measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb (orange, green, red, respectively).
	Animal-Free™ Recombinant Human KGF/FGF‑7 Protein Bioactivity. Animal-Free™ Recombinant Human KGF/FGF-7 Protein (Catalog # BT-KGF-AFL) induced cell proliferation in Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
	Animal-Free™ Recombinant Human KGF/FGF‑7 Protein SDS-PAGE. 2 μg/lane of Animal-Free™ Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-KGF-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: KGF/FGF-7

Keratinocyte Growth Factor (KGF), or Fibroblast Growth Factor-7 (FGF-7), is an important component in many cell culture protocols. It is widely used to promote the growth and differentiation of epithelial cells, including skin keratinocytes, and lung, corneal, and intestinal epithelia. It is a part of tissue engineering and organoid culture protocols, where it can enhance the generation of complex tissues such as liver, gastrointestinal tract, lung, and mammary gland. Furthermore, KGF/FGF-7 has been shown to stimulate the proliferation of pancreatic endocrine progenitor cells and promote their differentiation into mature insulin-secreting beta cells. In these capacities KGF/FGF-7 has significant clinical relevance in studies to better understand gastrointestinal disorders, wound healing, respiratory disease, and diabetes.

Item	Value
Long Name	Keratinocyte Growth Factor
Entrez Gene IDs	2252 (Human); 403915 (Canine)
Alternate Names	FGF-7, FGF7, HBGF-7, HBGF7, Heparin-binding growth factor 7, KGF, KGFfibroblast growth factor 7 (keratinocyte growth factor), fibroblast growth factor 7HBGF-7, keratinocyte growth factor

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Customer Reviews for Recombinant Human KGF/FGF-7 Protein, Animal-Free

There are currently no reviews for this product. Be the first to review Recombinant Human KGF/FGF-7 Protein, Animal-Free and earn rewards!

Have you used Recombinant Human KGF/FGF-7 Protein, Animal-Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image