Recombinant Human IL-17E Protein

Name: Recombinant Human IL-17E Protein
Brand: R&D Systems
SKU: 1258-IL
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1258-IL

Citations (20)

(2)

Product Datasheet / COA / SDS

New Version Available - 8134-IL/CF

A New and Improved rh IL-17E is Now Available! The new protein is mammalian cell expressed!

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	Q9H293
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-17E Protein

Product Specifications for Recombinant Human IL-17E Protein

Source	E. coli-derived human IL-17E/IL-25 protein Tyr33-Gly177, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17 kDa (monomer)
Activity	Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.25-1.5 ng/mL.

Scientific Data Examples for Recombinant Human IL-17E Protein

	Recombinant Human IL-17E Protein Bioactivity Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.25-1.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1258-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1258-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17E/IL-25

The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1- and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL‑25 shares 80% amino acid sequence identity with mouse and rat IL‑25 (3, 4). During helminth infections and allergic reactions, IL‑25 is locally up‑regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma, IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4⁺ T cell infiltration into the brain (17).

Item	Value
References	Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781. Iwakura, Y. et al. (2011) Immunity 34:149. Lee, J. et al. (2001) J. Biol. Chem. 276:1660. Fort, M.M. et al. (2001) Immunity 15:985. Zhao, A. et al. (2010) J. Immunol. 185:6921. Suzukawa, M. et al. (2012) J. Immunol. 189:3641. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579. Petersen, B.C. et al. (2012) Nat. Med. 18:751. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837. Rickel, E.A. et al. (2008) J. Immunol. 181:4299. Hurst, S.D. et al. (2002) J. Immunol. 169:443. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229. Stock, P. et al. (2009) J. Immunol. 182:5116. Caruso, R. et al. (2009) Gastroenterology 136:2270. Goswami, S. et al. (2009) Nat. Immunol. 10:496. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
Long Name	Interleukin 17E
Entrez Gene IDs	64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names	IL-17E, IL-25, IL17E, IL25, Interleukin-17E, interleukin 25, interleukin-25

Citations for Recombinant Human IL-17E Protein (20)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-17E Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Xiao et al. (2022-01-01), IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 41(12):111848.
PMID: 36543124
Species: Human
Sample Types: Cells
Applications: Cell Culture

T Petrova et al. (2020-01-01), p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells Sci Rep, 10(1):3479.
PMID: 32103032
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Hochdörfer et al. (2019-01-01), Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 0(0).
PMID: 30892687
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Qin et al. (2019-01-01), The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 117(0):84-90.
PMID: 30831444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Tang et al. (2018-01-01), Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma Clin Transl Allergy, 8(0):5.
PMID: 29456832
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Lombardi et al. (2017-01-01), Effector and regulatory dendritic cells display distinct patterns of miRNA expression Immun Inflamm Dis, 0(0).
PMID: 28497578
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ling Y et al. (2015-01-01), Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med, 212(5):619-31.
PMID: 25918342
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yao et al. (2015-01-01), IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model. Respir Res, 16(0):39.
PMID: 25889697
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wu L et al. (2014-01-01), The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol, 193(4):1590-9.
PMID: 25024377
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Biancheri P et al. (2014-01-01), Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 44(2):370-85.
PMID: 24338958
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Teunissen M et al. (2014-01-01), Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients. J Invest Dermatol, 134(9):2351-60.
PMID: 24658504
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Boisson B et al. (2013-01-01), An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis. Immunity, 39(4):676-86.
PMID: 24120361
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hvid M et al. (2011-01-01), IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction? J. Invest. Dermatol., 131(1):150-7.
PMID: 20861853
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xu G et al. (2010-01-01), Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 65(5):581-9.
PMID: 19968632
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Terrier B et al. (2010-01-01), Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome. Blood, 116(22):4523-31.
PMID: 20729468
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Duluc D et al. (2009-01-01), Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 125(2):367-73.
PMID: 19378341
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cherry WB et al. (2008-01-01), A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. J. Allergy Clin. Immunol., 121(6):1484-90.
PMID: 18539196
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rickel EA et al. (2008-01-01), Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 181(6):4299-310.
PMID: 18768888
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LÃƒÂ©tuvÃƒÂ© S et al. (2006-01-01), IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts. J. Allergy Clin. Immunol., 117(3):590-6.
PMID: 16522458
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wong CK et al. (2005-01-01), Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB. Am. J. Respir. Cell Mol. Biol., 33(2):186-94.
PMID: 15860795

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Customer Reviews for Recombinant Human IL-17E Protein (2)

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Recombinant Human IL-17E Protein

Name: Anonymous

Application: ELISA standard

Verified Customer | Posted 06/09/2020

We used this protein in an in-house ELISA along with mAb (1258) and pAb (AF1258) to quantify IL-17E in human serum and plasma. This combination detected IL-17E in our samples efficiently.
Recombinant Human IL-17E Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Reason for Rating: This recombinant protein works very well in vitro assay.

Verified Customer | Posted 07/29/2016

Recombinant Human IL-17E Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-17E Protein

Scientific Data Examples for Recombinant Human IL-17E Protein

Formulation, Preparation and Storage

Carrier Free

1258-IL

Carrier

1258-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-17E/IL-25

Citations for Recombinant Human IL-17E Protein (20)

Customer Reviews for Recombinant Human IL-17E Protein (2)

Customer Images

Recombinant Human IL-17E Protein

Recombinant Human IL-17E Protein

Product Documents for Recombinant Human IL-17E Protein

Product Datasheets

COA

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SDS

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