Recombinant Human IL-17C Protein

Name: Recombinant Human IL-17C Protein
Brand: R&D Systems
SKU: 1234-IL
Rating: 5 (1 reviews)

Catalog # 1234-IL | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (4)

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	Q9P0M4.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human IL-17C Protein

Technical Data
Citations (4) / Reviews (1)

Product Specifications for Recombinant Human IL-17C Protein

Source	E. coli-derived human IL-17C protein His19-Val197, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	19.8 kDa (monomer)
SDS-PAGE	19.8 kDa (monomer)
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17 RE Fc Chimera (Catalog # 8358-MR) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human IL-17C that produces 50% of the optimal binding response is 1.5-7.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1234-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1234-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17C

Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17C shares 79% and 76% amino acid sequence identity with mouse and rat IL‑17C, respectively (3). IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity (4, 5). These two receptor chains can associate into a heterodimeric receptor for IL‑17C (4‑6). IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells, while IL‑17 RA is widely expressed (4, 5). IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa (1, 2). IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4⁺ T cells, macrophages, and dendritic cells (4, 6‑9). It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL‑17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20 (5).

Item	Value
References	Pappu, R. et al. (2012) Trends Immunol. 33:343. Rubino, S.J. et al. (2012) Trends Immunol. 33:112. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773. Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159. Chang, S.H. et al. (2011) Immunity 35:611. Song, X. et al. (2011) Nat. Immunol. 12:1151. Pfeifer, P. et al. (2012) Am. J. Respir. Cell Mol. Biol. 48:415. Johnston, A. et al. (2013) J. Immunol. 190:2252. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128. Johansen, C. et al. (2009) Br. J. Dermatol. 160:319. Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
Long Name	Interleukin 17C
Entrez Gene IDs	27189 (Human); 234836 (Mouse)
Alternate Names	CX2, Cytokine CX2, IL-17C, IL-17CMGC126884, IL17C, MGC138401, interleukin 17C, interleukin-17C

Citations for Recombinant Human IL-17C Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-17C Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Xiao et al. (2022-12-20), IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-141(12):111848.
PMID: 36543124
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

F Ritzmann et al. (2019-07-17), IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer Sci Rep, 2019-09(1):10353.
PMID: 31316109
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johnston A et al. (2013-01-28), Keratinocyte overexpression of IL-17C promotes psoriasiform skin inflammation. J Immunol, 2013-0190(5):2252-62.
PMID: 23359500
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448

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Customer Reviews for Recombinant Human IL-17C Protein (1)

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Recombinant Human IL-17C Protein

Name: Rachel Levantovsky

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 07/27/2020

Recombinant Human IL-17C Protein

Catalog # 1234-IL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human IL-17C Protein

Formulation, Preparation and Storage

Carrier Free

1234-IL

Carrier

1234-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-17C

Citations for Recombinant Human IL-17C Protein (4)

Customer Reviews for Recombinant Human IL-17C Protein (1)

Recombinant Human IL-17C Protein

Product Documents for Recombinant Human IL-17C Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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