Recombinant Human IL-1 RII Protein, CF

Catalog # 263-2R/CF | R&D Systems, Inc. a Bio-Techne Brand
A New rh IL-1 RII is Available! ~3 fold better activity; Lower endotoxin specification; CHO expressed; His tag!
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263-2R-050/CF
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Key Product Details

Accession #

P27930

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-1 RII protein
Phe14-Glu343

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe14

Predicted Molecular Mass

36 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-1 beta-dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
Approximately 1-5 µg/mL of IL- sRII will inhibit 50% of the biological response due to 50 pg/mL of recombinant human IL-1 beta.

Formulation, Preparation and Storage

263-2R/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein (1) that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified. This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor II

Alternate Names

CD121b, IL-1 R beta, IL-1RII, IL1R2, IL1RII

Entrez Gene IDs

7850 (Human); 16178 (Mouse)

Gene Symbol

IL1R2

UniProt

P27930

Product Documents for Recombinant Human IL-1 RII Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-1 RII Protein, CF

For research use only

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