Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP
R&D Systems, part of Bio-Techne | Catalog # 201-GMP
Animal-Free.
Key Product Details
Product Specifications
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.
Ala117-Ser269
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.
Purity
>97%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala-Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu
Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu-Arg
Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu-Arg
Predicted Molecular Mass
17 kDa
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is >5.0 x 107 IU/mg, which is calibrated against the human IL-1 beta WHO International Standard (NIBSC code: 86/680).
The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is >5.0 x 107 IU/mg, which is calibrated against the human IL-1 beta WHO International Standard (NIBSC code: 86/680).
Host Cell Protein
< 0.5 ng per µg of protein when tested by ELISA.
Mycoplasma
Negative for Mycoplasma.
Scientific Data Images for Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF
Recombinant Human IL-1 beta/IL-1F2 GMP Protein Bioactivity
GMP-grade Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-GMP) stimu-lates proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
Recombinant Human IL-1 beta/IL-1F2 GMP Protein SDS-PAGE
1 μg/lane of GMP-grade Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta/IL-1F2 GMP Protein Mass Spectrometry
ESI analysis of GMP-grade Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-GMP). The labeled peaks at 17376 Da and 17305 Da correspond to the calculated molecular masses, 17377 Da (aa 117-269) and 17306 Da (aa 118-269), respectively.Formulation, Preparation and Storage
201-GMP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100-200 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta-converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- March, C.J. et al. (1985) Nature 315:641.
- Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF
Manufacturing Specifications
GMP ProteinsR&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
R&D Systems' quality focus includes:
- Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
- Documented processes and QA control of documentation and process changes
- Personnel training programs
- Raw material testing and vendor qualification/monitoring
- Fully validated equipment, processes and test methods
- Equipment calibration schedules using a computerized calibration program
- Facility maintenance, safety programs and pest control
- Material review process for variances
- Monitoring of stability over product shelf-life
- N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
- Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
- Host Cell Protein testing performed by ELISA
- Mycoplasma testing by ribosomal RNA hybridization assay
Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.
R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.
Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
Product Specific Notices for Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF
Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: This product is covered by one or more of the following U.S. patents: 5,681,933 and5,474,899.
For preclinical, or clinical ex vivo use
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