Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Name: Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11574-AB

R&D Systems, part of Bio-Techne | Catalog # 11574-AB

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Product Datasheet / COA / SDS

Key Product Details

Source:	HEK293
Structure / Form:	Disulfide linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human IFN-alpha/beta R1 protein

Human IFNAR1 (Lys28-Lys436) Accession # AAA52730.1	GGIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

74 kDa

SDS-PAGE

74 kDa

Activity

Measured by its binding ability in a functional ELISA.

Recombinant Human IFN-alpha/beta R1 Fc Chimera (Catalog # 11574-AB) binds Recombinant Human LRPAP (Catalog # 4296-LR) with an ED₅₀ of 0.750-9.00 μg/mL.

Scientific Data Examples for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

	Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein Binding Activity. Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein (Catalog # 11574-AB) binds Recombinant Human LRPAP (4296-LR) with an ED₅₀ of 0.750-9.00 μg/mL.
	Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein (Catalog # 11574-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa and 210-240 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11574-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-alpha/beta R1

Interferon‑alpha/beta receptor 1 (IFN‑ alpha / beta R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN‑ alpha / beta R1, in association with IFN‑ alpha / beta R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta (2, 3). Mature human IFN‑ alpha / beta R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN‑ alpha / beta R1 shares 47% and 50% aa identity with mouse and rat IFN‑ alpha / beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN‑ alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha / beta R2. Ligands preferentially associate with IFN‑ alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN‑ alpha / beta R1 (5‑7). IFN‑ alpha / beta R1 also associates with IFN‑ gamma R2 even in the absence of IFN‑ gamma stimulation (3). IFN‑ alpha / beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down‑regulation of the receptor is accomplished by ligand‑dependent or ‑independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10‑13).

Item	Value
References	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284. Takaoka, A. et al. (2000) Science 288:2357. Uze, G. et al. (1990) Cell 60:225. Lamken, P. et al. (2004) J. Mol. Biol. 341:303. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867. Kalie, E. et al. (2008) J. Biol. Chem. 283:32925. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375. Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328. Zheng, H. et al. (2011) Blood 118:4003. Qian, J. et al. (2011) PLoS Pathogens 7:e1002065. Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318. Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.
Long Name	Interferon alpha/beta Receptor 1
Entrez Gene IDs	3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names	AVP, CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-aR1, IFN-alpha-REC, IFN-alpha/beta R1, IFN-alpha/beta receptor 1, IFN-bR1, IFNAR, IFNAR1, IFNBR, IFNbR1, Type I interferon receptor 1, alpha-type antiviral protein, beta-type antiviral protein, human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC, interferon (alpha, beta and omega) receptor 1, interferon alpha/beta receptor 1, interferon-alpha/beta receptor alpha chain, interferon-beta receptor 1

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Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11574-AB

Key Product Details

Product Specifications for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IFN-alpha/beta R1

Customer Reviews for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Product Documents for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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