Recombinant Human His6-USP13 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-588
Discontinued Product
E-588 has been discontinued.
View all USP13 products.
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP13 protein
Contains an C-terminal 6-His tag
Contains an C-terminal 6-His tag
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
98 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Formulation, Preparation and Storage
E-588
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP13
Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages. Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.
References
- Xiang, S. et al. (2015) Oncol Rep. 33: 1342
- Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
- Zhang, Y-H. et al. (2011) PLoS One 6: e29362
- Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421
Long Name
Ubiquitin Specific Protease 13
Alternate Names
Isopeptidase T-3, IsoT-3, Ubiquitin Thioesterase 13
Gene Symbol
USP13
UniProt
Additional USP13 Products
Product Documents for Recombinant Human His6-USP13 Protein, CF
Product Specific Notices for Recombinant Human His6-USP13 Protein, CF
For research use only
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