Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E2-630

 
R&D Systems, part of Bio-Techne
Discontinued Product
E2-630 has been discontinued. View all UbcH6/UBE2E1 products.

Key Product Details

Source

E. coli

Accession #

P51965

Conjugate

Unconjugated

Applications

Enzyme Activity

View all UbcH6/UBE2E1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UbcH6/UBE2E1 protein
Contains an N-terminal Gly-Ser-Ser and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

23 kDa

Activity

Recombinant Human His6-UbcH6/UBE2E1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH6/UBE2E1 concentration of 0.1-1 μM.

Formulation, Preparation and Storage

E2-630
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UbcH6/UBE2E1

Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins (1). UbcH6/UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136 (2). In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination (3,4). UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection (5). In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia (6,7).

References

  1. Nuber, U. et al. (1996) J. Biol. Chem. 271: 2795.

  2. Takeuchi, T. et al. (2005) J. Biochem. 138:711.

  3. Espinosa, A. et al. (2008) Exp. Cell Res. 314:3605.

  4. Espinosa, A. et al. (2011) J. Biol. Chem. 286:36478.

  5. Vanni, E. et al. (2012) J. Virol. 86:6323.

  6. Hong, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:256.

  7. Lee, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:735.

  Additional Sources
  1. Ito K., et al. (2001) Eur J. Biochem. 268: 2725-2732
  2. Nuber U., et al. (1996) J. Biol. Chem. 271: 2795-800
  3. Plafker S.M., et al. (2004) J. Cell Biol. 167: 649-659
  4. Rajendra R., et al. (2004) J. Biol. Chem. 279: 36440-36444
  5. Vichi A., et al. (2005) Proc. Natl. Acad. Sci. 102: 1945-1950
  6. Wang Z., et al. (2005) J. Virol. 79: 8764-8772
 

Alternate Names

UBE2E1

Entrez Gene IDs

7324 (Human); 22194 (Mouse)

Gene Symbol

UBE2E1

UniProt

P51965 (Human)

Additional UbcH6/UBE2E1 Products

Product Documents for Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

For research use only

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