Key Product Details
Source
Sf 21 (baculovirus)
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP47 protein
Contains an N-terminal 10-His tag
Contains an N-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
160 kDa
Activity
Recombinant Human USP47 is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 10-100 nM USP47 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
Reviewed Applications
Read 1 review rated 5 using E-626 in the following applications:
Formulation, Preparation and Storage
E-626
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: USP47
References
- Palazon-Riquelme, P. et al. (2018) EMBO Rep. doi: 10.15252/embr.201744766
- Yu, L. et al. (2019) Cancer Letters doi: 10.1016/j.canlet.2019.01.039
Long Name
Ubiquitin Specific Protease 47
Alternate Names
TRFP
Gene Symbol
USP47
Additional USP47 Products
Product Documents for Recombinant Human His10-USP47 Protein, CF
Product Specific Notices for Recombinant Human His10-USP47 Protein, CF
For research use only
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