Recombinant Human His10-USP24 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-616

R&D Systems, part of Bio-Techne
Discontinued Product
E-616 has been discontinued. View all USP24 products.

Key Product Details

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

View all USP24 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP24 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

296 kDa

Activity

Recombinant human USP24 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP24 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine 110 substrates.

Formulation, Preparation and Storage

E-616
Formulation X mg/ml (X μM) is 50 mM HEPES, pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT, 1 mM EDTA
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP24

USP24 is a deubiquitinating enzyme (DUB) of the C19 peptidase family.  Human USP24 has a predicted molecular weight of 294 kDa and is 98% identical to the mouse orthologue.  USP24 reportedly deubiquitinates a wide variety of proteins such as p300, Bax, E2F4, securin, p53 and Mcl-1.  In one study, decreased USP24 activity correlated with decreased cell apoptosis and increased cell cycle progression seen in samples from clinical lung cancer patients.  Conversely, a compensatory upregulation of USP24 activity resuced myeloma cells from apoptosis when USP9x activity was reduced via RNA-mediated knockdown or chemical inhibition with WP1130.  Finally, USP24 is upregulated by DNA damaging agents and plays an important role in maintaining genome stability through its deubiquitination and stabilization of p53.  This recombinant enzyme cleaves all poly-Ubiquitin chain linkages in vitro, and contains a c-terminal 10-His tag.

References

  1. Peterson L.F. et al.  (2015) Blood  125:  3588
  2. Wang S.A. et al.  (2017)  Oncogene  36:  2930
  3. Zhang L. & Gong F.  (2016)  Mol. Cell Oncol.  3:  e1011888

Long Name

Ubiquitin Specific Protease 24

Alternate Names

Deubiquitinating enzyme 24, EC 3.4.19.12, FLJ31309, KIAA1057ubiquitin carboxyl-terminal hydrolase 24, ubiquitin specific peptidase 24, ubiquitin specific protease 24, ubiquitin thioesterase 24, Ubiquitin thiolesterase 24, ubiquitin-specific processing protease 24, Ubiquitin-specific-processing protease 24

Entrez Gene IDs

23358 (Human); 329908 (Mouse); 313427 (Rat)

Gene Symbol

USP24

Additional USP24 Products

Product Documents for Recombinant Human His10-USP24 Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His10-USP24 Protein, CF

For research use only

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