Recombinant Human FGFR4 His-tag Protein, CF

Name: Recombinant Human FGFR4 His-tag Protein, CF
Brand: R&D Systems
SKU: 11120-FR

Catalog # 11120-FR | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P22455.2
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGFR4 His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human FGFR4 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human FGFR4 protein Leu22-Asp369, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu22
Predicted Molecular Mass	39 kDa
SDS-PAGE	39 kDa
Activity	Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (Catalog # 232-FA), Human FGFR4 His-tag Protein binds with an ED₅₀ of 30.0-180 ng/mL.

Scientific Data Examples for Recombinant Human FGFR4 His-tag Protein, CF

	Recombinant Human FGFR4 His-tag Protein Bioactivity. In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Recombinant Human FGFR4 His-tag Protein (Catalog # 11120-FR) binds with an ED₅₀ of 30.0‑180 ng/mL.
	Recombinant Human FGFR4 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human FGFR4 His-tag Protein (Catalog # 11120-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11120-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR4

Fibroblast growth factor receptor 4 (FGFR4) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing and extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain (1, 2). The ECD of mature human FGFR4 shares 90% amino acid sequence identity with mouse FGFR4. Alternative splicing of the IgIII domain generates multiple forms of FGFR1-3, but FGFR4 does not have a splice variant (3, 4). FGFR4 exhibits distinct and varying binding affinities for different FGF ligands, with FGF1, FGF4, and FGF8 showing the highest affinity (4). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR4 is expressed at high levels during embryonic development and is required for the maintenance of both lipid and glucose metabolism as well as an established role in cholesterol metabolism (7). Overexpression of the FGFR4 has been reported in several solid tumors including breast cancer, prostate cancer, pancreatic cancer, and renal cell carcinoma (4, 8). Further, FGFR4 expression is significantly upregulated in most liver cancer cases, and enhanced FGF19-FGFR4 signaling is linked to hepatocellular carcinoma progression, metastasis, and poor survival (8). FGFR4 is being explored as a potential therapeutic target for breast cancer and other solid tumors (9).

Item	Value
References	Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev Dev, Biol. 4:215. Zhang, X. et al. (2006) J Biol Chem. 281:15694. Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201. Lang, L. and Teng, Y. (2019) Cells. 8:31. Xie, Y. et al. (2020) Sig Transduct Target Ther 5:181. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79. Huang, X. et al. (2007) Diabetes 56:2501. Liu, Y. et al. (2020) Front Cell Dev Biol. 8:95. Levine, K.M. et al. (2020) Pharmacol Ther. 214:107590.
Long Name	Fibroblast Growth Factor Receptor 4
Entrez Gene IDs	2264 (Human); 14186 (Mouse)
Alternate Names	CD334, CD334 antigen, EC 2.7.10, EC 2.7.10.1, FGF R4, FGFR-4, FGFR4, JTK2hydroxyaryl-protein kinase, MGC20292, TKF, fibroblast growth factor receptor 4, protein-tyrosine kinase, tyrosine kinase related to fibroblast growth factor receptor, tyrosylprotein kinase

Citations for Recombinant Human FGFR4 His-tag Protein, CF

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Recombinant Human FGFR4 His-tag Protein, CF

Catalog # 11120-FR | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human FGFR4 His-tag Protein, CF

Scientific Data Examples for Recombinant Human FGFR4 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGFR4

Citations for Recombinant Human FGFR4 His-tag Protein, CF

Product Documents for Recombinant Human FGFR4 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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