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Recombinant Human EBI3 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11084-EI

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11084-EI-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein
Arg21-Lys220

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

23 kDa

SDS-PAGE

30-40 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

Scientific Data Images for Recombinant Human EBI3 Protein, CF

Recombinant Human EBI3 Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

Recombinant Human EBI3 Protein SDS-PAGE.

2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.

Formulation, Preparation and Storage

11084-EI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI3

EBI3 (Epstein-Barr virus-induced gene 3 protein) also known as IL-27B, is a secreted cytokine (1). It shares similar domain structure as IL-12B except there are only two fibronectin type -III domains instead of three. Through non-covalent interaction, EBI-3 binds to IL-12A (IL-12 p35), IL-23A (IL-23p19), or IL-27A (IL-27p28/IL-30) to form IL-35, IL-39, or IL-27, respectively (2-4). In addition to forming the heterodimeric cytokines, EBI-3 can also function on its own. Expression of EBI-3 is induced in neutrophils stimulated with TLR8 ligands, with no detectable increase in IL-35, IL-39 or IL-27 (5). EBI-3 also mediate IL-6 trans-signaling through receptor gp130 in B9 mouse plasmacytoma cells (6).

References

  1. Devergne, O. et al. (1996) J. Virol. 70:1143.
  2. Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
  3. Pflanz, S. et al. (2002) Immunity 16:779.
  4. Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
  5. Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
  6. Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Gene Symbol

EBI3

UniProt

Additional EBI3 Products

Product Documents for Recombinant Human EBI3 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human EBI3 Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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