Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

Name: Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 666-DN
Price: 405 USD

Catalog # 666-DN | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q15762
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

Product Specifications for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human DNAM-1/CD226 protein

Human DNAM-1/CD226 (Glu19-Asn247) Accession # Q15762	HIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu19

Predicted Molecular Mass

53 kDa (monomer)

SDS-PAGE

75-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human DNAM-1/CD226Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human CD155/PVR Fc Chimera (Catalog # BT9174) with an ED₅₀ of 3-18 ng/mL.

Scientific Data Examples for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

	Recombinant Human DNAM-1/CD226 Fc Chimera Protein Binding Activity When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 1 µg/mL, 100 µL/well, Biotinylated Recombinant Human CD155/PVR (Catalog # BT9174) binds with an ED₅₀ of 3-18 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

666-DN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12-14).

Item	Value
References	Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359. Shibuya, A. et al. (1996) Immunity 4:573. Bottino, C. et al. (2003) J. Exp. Med. 198:557. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533. Dardalhon, V. et al. (2005) J. Immunol. 175:1558. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491. Morimoto, K. et al. (2007) Oncogene July 16 epub. Shibuya, K. et al. (1999) Immunity 11:615. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name	DNAX Accessory Molecule 1
Entrez Gene IDs	10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names	CD226, CD226 antigenplatelet and T cell activation antigen 1, CD226 molecule, DNAM-1, DNAM-1DNAX accessory molecule-1, DNAM1, DNAM1adhesion glycoprotein, DNAX accessory molecule 1, PTA1, T lineage-specific activation antigen 1 antigen, TLiSA1

Citations for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF (4)

Showing 1 - 4 of 4 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

P Fittje et al. (2022-06-24), HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells Oncogene, 2022-018(6):e1010572.
PMID: 35749424
Species: Human
Sample Types: Transduced Whole Cells
Applications: Flow Cytometry

J Joung et al. (2022-03-25), CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-013(1):1606.
PMID: 35338135
Species: Human
Sample Types: Serum
Applications: ELISA Standard

M Nakano et al. (2021-08-09), Association of elevated serum soluble CD226 levels with the disease activity and flares of systemic lupus erythematosus Scientific Reports, 2021-011(1):16162.
PMID: 34373559
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Oda T et al. (2004-07-09), Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts. Biochem. Biophys. Res. Commun., 2004-0319(4):1253-64.
PMID: 15194502

There are no citations that match your criteria.

Showing 1 - 4 of 4 citations Showing All

Product Documents for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS