Recombinant Human CD74 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3590-CDB

Analyzed by SEC-MALS
R&D Systems, part of Bio-Techne
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3590-CDB-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD74 Protein (3590-CDB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

NP_004346.1

Conjugate

Unconjugated

Applications

Bioactivity

View all CD74 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD74 protein
Gln73-Met232, with an N-terminal HA (YPYDVPDYA) tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (of HA tag)

Predicted Molecular Mass

19 kDa

SDS-PAGE

25-33 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human CD74 (Catalog # 3590-CDB) binds to Human CD74 Antibody (Catalog # AF3590) with an ED50 of 0.300-3.60 ng/mL.

Scientific Data Images for Recombinant Human CD74 Protein, CF

Recombinant Human CD74 Protein SEC-MALS.

Recombinant Human CD74 Protein (Catalog # 3590-CDB) has a molecular weight (MW) of 67.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human CD74 Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human CD74 (Catalog # 3590-CDB) binds to Human CD74 Antibody (Catalog # AF3590) with an ED50 of 0.300-3.60 ng/mL.

Recombinant Human CD74 Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD74 Protein (Catalog # 3590-CDB) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 25-33 kDa.

Formulation, Preparation and Storage

3590-CDB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature human CD74 consists of a 46 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2, 3). Alternate splicing and the use of a second initiation start site result in the synthesis of additional isoforms (ranging from 31 kDa ‑ 41 kDa) that differ by the presence or absence of a 16 aa N‑terminal extension and/or a 64 aa internal section of the ECD (4). This recombinant protein does not contain the 64 aa insertion. Within comparable regions of the ECD, human CD74 shares 70% and 73% aa sequence identity with mouse and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (5). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (6, 7). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (8 ‑ 13). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (12). CD74 is up‑regulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (14, 15). It is also up‑regulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 16).

References

  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Claesson, L. et al. (1983) Proc. Natl. Acad. Sci. 80:7395.
  3. Strubin, M. et al. (1984) EMBO J. 3:869.
  4. Strubin, M. et al. (1986) EMBO J. 5:3483.
  5. Riberdy, J.M. et al. (1992) Nature 360:474.
  6. Koch, N. et al. (1991) J. Immunol. 147:2643.
  7. Roche, P.A. et al. (1991) Nature 354:392.
  8. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  9. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  10. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
  11. Shi, X. et al. (2006) Immunity 25:595.
  12. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  13. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  14. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  15. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  16. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Gene Symbol

CD74

UniProt

NP_004346.1

Additional CD74 Products

Product Documents for Recombinant Human CD74 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD74 Protein, CF

For research use only

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