Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT10961

Fc Chimera
R&D Systems, part of Bio-Techne
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BT10961-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD117/c-kit Fc Biotinylated Protein (BT10961)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all CD117/c-kit Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein
Human CD117/c-kit
(Gln26-Thr520)
Accession # P10721.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26

Predicted Molecular Mass

82 kDa

SDS-PAGE

110-120 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human CD117/c-kit Fc Chimera (Catalog # BT10961) binds Recombinant Human SCF/c-kit Ligand (Catalog # BT-SCF) with an ED50 of 0.400-4.00 μg/mL.

Scientific Data Images for Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF

Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein Binding Activity.

Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # BT10961) binds Recombinant Human SCF/c-kit Ligand (BT-SCF) with an ED50 of 0.400-4.00 μg/mL.

Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # BT10961) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-120 kDa and 220-240 kDa, respectively.

Formulation, Preparation and Storage

BT10961
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF. Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1-3). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. Several SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and can be characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (4, 5). Binding of SCFR to SCF triggers receptor dimerization and activates downstream signaling (6). SCF is a primary growth and activation factor for mast cells and eosinophils and SCFR expression on mast cells enables them to infiltrate SCF-secreting tumors where they promote tumor growth and induce local immune suppression (7, 8). SCFR is up regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (9). SCFR protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (10, 11). Mutations or deletions of SCFR cause a wide variety of malignancies as well as pigmentation disorders and sterility (12).

References

  1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
  2. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  3. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  4. Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
  5. Caruana, G. et al. (1999) Oncogene 18:5573.
  6. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  7. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  8. Huang, B. et al. (2008) Blood 112:1269.
  9. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  10. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  11. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  12. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF

For research use only

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