Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT8918

Butyrophilin 2A2 Learn More About B7-related Butryophilins as Immune Modulators
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Discontinued Product
BT8918 has been discontinued. View all BTN2A2/Butyrophilin 2A2 products.

Key Product Details

Source

HEK293

Accession #

Q8WVV5

Structure / Form

Disulfide-linked homodimer. Biotinylated via sugars

Conjugate

Biotin

Applications

Bioactivity

View all BTN2A2/Butyrophilin 2A2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTN2A2/Butyrophilin 2A2 protein
Human BTN2A2
(Gln33-Val237)
Accession # Q8WVV5		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No result obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Phe34

Predicted Molecular Mass

50 kDa (unlabeled)

SDS-PAGE

66-77 kDa and 140-180 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein, CF

Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein Bioactivity

Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein Bioactivity

Both Biotinylated Recombinant Human BTN2A2 Fc Chimera (Catalog # BT8918) and Recombinant Human BTN2A2 Fc Chimera (Catalog # 8918-BT) inhibit anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.1-0.5 µg/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

BT8918
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature human Butyrophilin 2A2 consisits of a 233 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 237 aa cytoplasmic domain. Within the Ig-like domains, human Butyrophilin 2A2 shares 70% aa sequence identity with mouse and rat Butyrophilin 2A2. Alternative splicing generates additional isoforms of human Butyrophilin 2A2 that lack the first, second, or both Ig-like domains as well as isoforms with substitutions and deletions in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
  3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
  4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.

Long Name

Butyrophilin Subfamily 2 Member A2

Alternate Names

BTF2, BTN2, Butyrophilin 2

Entrez Gene IDs

10385 (Human); 238555 (Mouse); 306957 (Rat)

Gene Symbol

BTN2A2

UniProt

Q8WVV5 (Human)

Additional BTN2A2/Butyrophilin 2A2 Products

Product Documents for Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human BTN2A2 Fc Chimera Biotinylated Protein, CF

For research use only

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