Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein Best Seller
Catalog # 5860-AP | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
HA (YPYDVPDYA) |
GCN4-IZ | GGGSGGGSGGGS | Human April (Ala105-Leu250) Accession # O75888 |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 5-25 ng/mL in the presence of goat anti-mouse IgM.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 5860-AP
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 5860-AP/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: APRIL/TNFSF13
References
- Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
- Yu, G. et al. (2000) Nat. Immunol. 1:252.
- Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
- Kelly, K. et al. (2000) Cancer Res. 60:1021.
- Day, E.S. et al. (2005) Biochemistry 44:1919.
- Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
- Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
- Schwaller, J. et al. (2007) Blood 109:331.
- Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
- Benson, M.J. et al. (2008) J. Immunol. 180:3655.
- He, B. et al. (2004) J. Immunol. 172:3268.
- Fernandez, L. et al. (2012) Ann. Rheum. Dis. 72:1367.
- Xiao, Y. et al. (2011) Eur. J. Immunol. 41:164.
- Sandberg, W.J. et al. (2009) Thromb. Haemost. 102:704.
- Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
Product Specific Notices for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
For research use only
Citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (10)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayCC Chan et al. (2021-05-18), A BAFF/APRIL axis regulates obesogenic diet-driven weight gain Nature Communications, 2021-012(1):2911.
PMID: 34006859 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Mulazzani et al. (2019-10-15), APRIL and BAFF: novel biomarkers for central nervous system lymphoma J Hematol Oncol, 2019-112(1):102.
PMID: 31615554 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayR Wilson et al. (2018-04-01), Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica Brain, 2018-0141(4):1063-1074.
PMID: 29447335 -
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPRAW Clarke et al. (2018-03-05), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-00(0):1-43.
PMID: 29436901 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayD Brach et al. (2017-08-23), EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL Mol. Cancer Ther., 2017-00(0).
PMID: 28835384 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySaulep-Easton D et al. (2015-07-03), The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-030(1):163-72.
PMID: 26139429 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMamara et al. (2015-04-09), TACI expression and signaling in chronic lymphocytic leukemia. J Immunol Res, 2015-02015(0):478753.
PMID: 25950010 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDeknuydt F et al. (2014-02-18), Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles. J Leukoc Biol, 2014-095(6):983-91.
PMID: 24550522 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayOsorio C et al. (2014-01-18), Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci, 2014-059(0):24-36.
PMID: 24444792 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayTai Y et al. (2013-04-16), CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications. Leukemia, 2013-028(1):155-65.
PMID: 23588715
There are no citations that match your criteria.
Customer Reviews for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (9)
Customer Images
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 02/19/2021
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Application: Cell migration/motilityVerified Customer | Posted 12/02/2020
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Application: Cell migration/motilityVerified Customer | Posted 10/15/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/16/2019Soluble APRIL enhances NGF-promoted axon growth from SCG neurons. (A) Images of representative calcein-labelled newborn SCG neurons incubated for 16 h in medium containing 0.1 ng/ml NGF and 25 µM Boc-D-FMK with and without 100 ng/ml soluble recombinant APRIL. Scale bar:100 µm. (B) Bar charts of total length of axons in the arbors of P0 SCG neurons grown for 16 h in medium containing 0.1 ng/ml NGF, 25 µM Boc-D-FMK and different concentrations of APRIL.
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Application: Cell AdhesionVerified Customer | Posted 12/06/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/06/2018
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Application: SDS-PAGE ControlVerified Customer | Posted 11/05/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/05/2018
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Application: Apoptosis assayVerified Customer | Posted 07/27/2018
There are no reviews that match your criteria.
FAQs for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
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Q: What is the meaning of "HA (YPYDVPDYA)" in the Source section of the datasheet?
A: HA (YPYDVPDYA) stands for Hemagglutinin, which consists of amino acids Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. It is a popular epitope tag in proteins with mammalian expression systems.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.