Recombinant Human alpha-Synuclein Low Endotoxin Protein , CF
R&D Systems, part of Bio-Techne | Catalog # SP-486
Discontinued Product
SP-486 has been discontinued.
View all alpha-Synuclein products.
Key Product Details
Product Specifications
Source
E. coli-derived human alpha-Synuclein protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
14 kDa
Activity
Recombinant Human alpha-Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application.
Scientific Data Images for Recombinant Human alpha-Synuclein Low Endotoxin Protein , CF
Recombinant Human alpha-Synuclein Low Endotoxin Protein SDS-PAGE
0.5 μg and 1 μg per lane of Recombinant Human alpha-Synuclein Low Endotoxin (Catalog # SP-486) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a single band at 19 kDa.Formulation, Preparation and Storage
SP-486
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: alpha-Synuclein
alpha-Synuclein is predominantly expressed in neurons of the central nervous system, specifically in the presynaptic region of nerve terminals. This protein cycles between a free, partially unfolded form and a helical, membrane-bound form. alpha-Synuclein can aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms are major components of Lewy Bodies and have been linked to the pathogenesis of Parkinson's disease dementia. alpha-Synuclein aggregates can also be found associated with amyloid plaques in Alzheimer's disease. This product has an endotoxin level < 0.01 EU per 1 μg of protein.
References
- Breydo L., Wu J.W., Uversky V.N. (2012) Biochim Biophys Acta 1822: 261
- Chen R.H. et al. (2013) J. Biol. Chem. 288: 7438
- Li X. et al. (2008) Acta. Biochim Biophys Sin (Shanghai) 40: 406
- Surguchov A. (2008) Int Rev Cell Mol. Biol. 270: 225
- Xia Q. et al. (2008) Front Biosci. 13: 3850
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
UniProt
Additional alpha-Synuclein Products
Product Documents for Recombinant Human alpha-Synuclein Low Endotoxin Protein , CF
Product Specific Notices for Recombinant Human alpha-Synuclein Low Endotoxin Protein , CF
For research use only
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