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Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF

Catalog # 10585-EB | R&D Systems, Inc. a Bio-Techne Brand
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10585-EB-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral EBOV GP protein
Ebola Virus GP1
(Ile33-Val311)
(Thr42Val, Thr230Val)
Accession # NP_0066246.1
Ebola Virus GP2
(Thr464-Asp632)
Accession # NP_066246.1
HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile33 & Glu502

Predicted Molecular Mass

51 kDa

SDS-PAGE

63-75 kDa, under non-reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Viral EBOV GP (mucin domain deleted, Catalog # 10585-EB) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CLEC10A/CD301 (Catalog # 4888-CL) binds with an ED50 of 1-10 ng/mL.

Scientific Data Images for Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF

Recombinant EBOV GP (mucin domain deleted) Protein Binding Activity.

When Recombinant Viral EBOV GP (Mucin Domain Deleted) Protein (Catalog # 10585-EB) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC10A/CD301 Protein (4888-CL) binds with an ED50 of 1-10 ng/mL.

Formulation, Preparation and Storage

10585-EB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

References

  1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
  2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
  3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
  4. Volchkov, V.E. et al. (1998) Virology 245:110.
  5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
  6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
  7. Takada, A. et al. (2004) J. Virol. 78:2943.
  8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
  9. Simmons, G. et al. (2003) Virology 305:115.
  10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
  11. Chandran, K. et al. (2005) Science 308:1643.
  12. Cote, M. et al. (2011) Nature 477:344.

Long Name

Ebola Virus Glycoprotein

Alternate Names

bovGP

Entrez Gene IDs

911829 (Viral)

Gene Symbol

GP

UniProt

Product Documents for Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF

For research use only

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