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Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF

Catalog # 10408-SR | R&D Systems, Inc. a Bio-Techne Brand
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10408-SR-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived IL-6R alpha protein
Cynomolgus Monkey/Rhesus Macaque
IL-6R alpha
(Leu20-Asp358)
Accession # XP_005541720.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

64 kDa

SDS-PAGE

89-101 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cynomolgus Monkey/Rhesus Macaque IL-6R alpha Fc Chimera (Catalog # 10408-SR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human IL-6 (Catalog # 7270-IL) binds with an ED50 of 7.5-45 ng/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF

Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein Binding Activity

Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein Binding Activity

When Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Chimera (Catalog # 10408-SR) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Human IL-6 (Catalog # 7270-IL) binds with an ED50 of 7.5-45 ng/mL.
Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-6R alpha Fc Chimera (Catalog # 10408-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 89-101 kDa and 170-190 kDa, respectively.

Formulation, Preparation and Storage

10408-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The cynomolgus IL-6R alpha cDNA encodes a precursor type I transmembrane protein of 468 amino acids (aa), and based on its similarity with human IL-6R alpha it is predicted to contain a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment likely contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, Cynomolgus IL-6R alpha shares 97% aa identity with human IL-6R alpha. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6R alpha. It has been documented that elevated soluble IL-6R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7).

References

  1. Yamasaki, K. et al. (1988) Science 241:825.
  2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
  3. Hibi, M. et al. (1990) Cell 63:1149.
  4. Saito, M. et al. (1992) J. Immunol. 148:4066.
  5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
  6. Jones, S.A. et al. (2001) FASEB J. 15:43.
  7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Long Name

Interleukin 6 Receptor alpha

Alternate Names

CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Gene Symbol

IL6R

UniProt

Product Documents for Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF

For research use only

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