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Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 7914-TM

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7914-TM-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey TIM-3 protein
Cynomolgus Monkey TIM-3
(Ser22-Arg201)
Accession # EHH54703
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

N-terminal Sequence Analysis

Ser22 & Val24

Predicted Molecular Mass

46.3 kDa (monomer)

SDS-PAGE

60-75 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.5-3 μg/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin‑9 (Catalog # 2045-GA) is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey TIM‑3 Chimera that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey TIM-3 (Catalog # 7914-TM) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Formulation, Preparation and Storage

7914-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM‑3 (T cell immunoglobulin and mucin domain‑3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig‑like V‑type domain and a Ser/Thr‑rich mucin stalk region (1, 2). Mature cynomolgus TIM‑3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. Within the ECD, cynomolgus (or crab‑eating macaque) monkey TIM‑3 shares 81%, 57%, and 56% aa sequence identity with human, mouse, and rat TIM‑3, respectively. TIM‑3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3‑10). Its binding to Galectin‑9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti‑tumor immunity (11). TIM‑3 ligation attenuates CD8+ and Th1 cell responses (11‑13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell‑expressed TIM‑3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (3). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (6). Soluble TIM‑3 is also reported to inhibit the response of T cells to both Ag‑induced and concurrent CD3/CD28 stimulation (15). By contrast, TIM‑3 interactions with Galectin‑9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References

  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Nakayama, M. et al. (2009) Blood 113:3821.
  4. Anderson, A.C. et al. (2007) Science 318:1141.
  5. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  6. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  7. Monney, L. et al. (2002) Nature 415:536.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
  15. Geng, H. et al. (2006) J. Immunol. 176:1411.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

UniProt

Additional TIM-3 Products

Product Documents for Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

For research use only

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