Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Name: Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11145-CD
Price: 405 USD

Catalog # 11145-CD | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P79185.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Product Specifications for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD4 protein

Cynomolgus Monkey CD4 (Lys26-Pro396) Accession # P79185.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Lys26

Predicted Molecular Mass

69 kDa

SDS-PAGE

80-88 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA with Human CD4 Biotinylated Antibody (Catalog # BAF379). The ED₅₀ for this effect is 35.0-280 ng/mL.

Scientific Data Examples for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

	Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein Binding Activity. Measured by its binding ability in a functional ELISA with Human CD4 Biotinylated Antibody (BAF379). The ED₅₀ for this effect is 35.0-280 ng/mL.
	Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein (Catalog # 11145-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-88 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11145-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature cynomolgus monkey CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, cynomolgus monkey and human CD4 shares approximately 91% aa sequence identity. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells (3). CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is re-expressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

Item	Value
References	Vignali, D.A.A. (2010) J. Immunol. 184:5933. Maddon, P.J. et al. (1985) Cell 42:93. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613. Biswas, P. et al. (2003) Blood 101:4452. Bernstein, H.B. et al. (2006) J. Immunol. 177:3669. Funke, I. et al. (1987) J. Exp. Med. 165:1230. Doyle, C. and J.L. Strominger (1987) Nature 330:256. Huppa, J.B. et al. (2010) Nature 463:963. Fragoso, R. et al. (2003) J. Immunol. 170:913. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109. Klatzmann, D. et al. (1984) Nature 312:767. Dagleish, A.G. et al. (1984) Nature 312:763.
Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names	CD4, CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, CD_antigen: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Catalog # 11145-CD | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD4

Citations for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Product Documents for Recombinant Cynomolgus Monkey CD4 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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