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Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

Catalog # 10052-AN | R&D Systems, Inc. a Bio-Techne Brand
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10052-AN-050

Key Product Details

Source

T. ni (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived cynomolgus monkey Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Glu460, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser17

Predicted Molecular Mass

53 kDa

SDS-PAGE

56-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
The IC50 for this effect is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

Recombinant Cynomolgus Angiopoietin-like 3 Protein Bioactivity

Recombinant Cynomolgus Angiopoietin-like 3 Protein Bioactivity

Recombinant Cynomolgus Monkey Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 10052-AN) dose dependently inhibits Recombinant Humam LPL (Catalog # 9888-LL) activity with an IC50 of 1.5-9 µg/mL.
Recombinant Cynomolgus Angiopoietin-like 3 Protein SDS-PAGE

Recombinant Cynomolgus Angiopoietin-like 3 Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Angiopoietin‑like Protein 3/ANGPTL3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Blue staining, showing bands at 56-65 kDa.

Formulation, Preparation and Storage

10052-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the Angiopoietins (1-3). Mature ANGPTL3 contains an N-terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do proteolytically separated N- and C-terminal fragments containing the coiled‑coil domain and fibrinogen-like domains, respectively (6, 7).ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alphaV beta3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver, also promotes the expansion of hematopoietic stem cells (16). Mature Cynomolgus Monkey ANGPTL3 shares 97%, 77%, and 78% amino acid sequence identity with human, mouse, and rat ANGPTL3, respectively.

References

  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Trans. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
  10. Koster, A. et al. (2005) Endocrinology 146:4943.
  11. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  12. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  13. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  14. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
  15. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  16. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Product Documents for Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

For research use only

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