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Recombinant Cotton Rat MIP-2 Protein

Catalog # 1084-M2 | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1084-M2-025/CF

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With Carrier
1084-M2-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived cotton rat CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala29-Asn101

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29

Predicted Molecular Mass

8.0 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2.
The ED50 for this effect is 3-15 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1084-M2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1084-M2/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage inflammatory protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79% and 85% aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR2 (1 - 5).

References

  1. Yoshie, O. et al. (2001) Adv. Immunol. 78:57.
  2. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  3. Telkamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.
  4. Lee, J. et al. (1995) J. Immunol. 155:2158.
  5. Feng, L. et al. (1995) J. Clin. Invest. 95:1009.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Gene Symbol

CXCL2

UniProt

Product Documents for Recombinant Cotton Rat MIP-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cotton Rat MIP-2 Protein

For research use only

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