Recombinant Human MMP-1 His Protein

Name: Recombinant Human MMP-1 His Protein
Brand: Novus Biologicals
SKU: NBP2-52617

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52617

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Accession #

NP_002412

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MMP-1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-469 of Human MMP-1

Source: Baculovirus

Amino Acid Sequence: HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNP RWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHF DEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAI TTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KNLEHHHHHH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

53.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MMP-1 His Protein

SDS-PAGE: Recombinant Human MMP-1 His Protein [NBP2-52617]

SDS-Page: Recombinant Human MMP-1 Protein [NBP2-52617]

Formulation, Preparation and Storage

NBP2-52617

Formulation	20 mM MES buffer (pH 5.5), 10 mM CaCl2, 100 mM NaCl, 0.05% Brij35, 30% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-1

MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Recombinant human MMP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Long Name

Matrix Metalloproteinase 1

Alternate Names

MMP1

Gene Symbol