Recombinant Mouse M-CSF Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35165

Novus Biologicals, part of Bio-Techne
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NBP2-35165-500ug
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Key Product Details

Source

E. coli

Accession #

Q3U4F9

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all M-CSF Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains corresponding to M-CSF Source: E. coli

Uniprot ID: Q3U4F9

Amino Acid Sequence: KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of M-CSF as determined by LAL method.

Predicted Molecular Mass

52 (homodimer) kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MCSF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35165
Formulation Lyophilized from a 0.2 um filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: M-CSF

Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229 a.a. of mature mouse M-CSF shares 87 %, 83 %, 82 % and 81 % sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Gene Symbol

CSF1

UniProt

Q3U4F9

Additional M-CSF Products

Product Documents for Recombinant Mouse M-CSF Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse M-CSF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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