I recently order guanosine deaminase protein (NBP2-23093) from your company. I need to find out what the expected enzyme activity is for this product in order to plan an experiment.

Name: Recombinant Human Guanine deaminase His Protein
Brand: Novus Biologicals
SKU: NBP2-23093

This protein has not been tested for activity and under the application section defines the only application this has been validated in as PAGE Poly-acrylamide Gel Electrophoresis as a control for western blot. When our proteins are tested and active, they will have a designation of FUNC (functional) in the Applications section. This does not mean the protein is not active but we have not tested or measured the protein activity and the process of purification did not necessarily conserve the activity.

Recombinant Human Guanine deaminase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23093

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Guanine deaminase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-454 of Human Guanine deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Guanine deaminase His Protein

SDS-PAGE: Recombinant Human Guanine deaminase His Protein [NBP2-23093]

SDS-Page: Recombinant Human Guanine deaminase Protein [NBP2-23093]

Formulation, Preparation and Storage

NBP2-23093

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Guanine deaminase

Guanine deaminase Protein is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human guanine deaminase Protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CYPIN, EC 3.5.4.3, GAH, GUANASE, Guanine aminase, Guanine aminohydrolase, guanine deaminase, KIAA1258, MGC9982, NEDASIN, p51-nedasin

Gene Symbol

GDA

Additional Guanine deaminase Products

Product Documents for Recombinant Human Guanine deaminase His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Guanine deaminase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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