Goat Keratan Sulfate Proteoglycans Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8618-KS

Isolated from goat cornea
R&D Systems, part of Bio-Techne
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8618-KS-010
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Key Product Details

  • R&D Systems Goat Cornea-derived Goat Keratan Sulfate Proteoglycans Protein (8618-KS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Goat Cornea

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Keratan Sulfate Proteoglycans Proteins and Enzymes »

Product Specifications

Source

Goat cornea-derived Keratan Sulfate Proteoglycans protein

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

30-300 kDa, reducing conditions

Activity

Measured by its ability to act as a substrate for Recombinant F. keratolyticus Endo-beta-galactosidase.
>90% of Keratan Sulfate Proteoglycans can be cleaved under the described conditions.

Formulation, Preparation and Storage

8618-KS
Formulation Supplied as a 0.2 μm filtered solution in deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Keratan Sulfate Proteoglycans

Keratan sulfate (KS) is a special glycosaminoglycan (GAG) found in cornea, cartilage and bone (1). GAGs are a group of linear polysaccharides with different repeating disaccharide units. Other GAGs include heparin, heparan sulfate (HS), chondroitin sulfate (CS), dermatan sulfate (DS), and hyaluronan (HA). Proteoglycans refer to GAG-protein conjugates. Known KS proteoglycans include lumican, keratocan, mimecan, aggrecan and fibromodulin. They have been identified in numerous epithelial and neural tissues in which KS expression responds to embryonic development, physiological variations, and wound healing (1). KS was first identified in 1939 by Suzuki in extracts of cornea (2), and later by Karl Meyer (3). The basic repeating disaccharide unit within KS is -3Gal beta1-4GlcNAc beta1 and are polymerized by B4GALT (4) and B3GNT families of glycosyltransferase (5). KS chains are subsequently sulfated by two specific sulfotransferases, CHST1 and CHST6, at a region close to its non-reducing end (6). KS is linked to core proteins either through O-glycan or N-glycan and corneal KS is mostly N-linked glycan (7). This product was purified from cornea of goat eyes through chemical extraction with 4M guanidine-HCl and further purified with anion-exchange chromatography steps. The identity of KS was confirmed by radioisotope 35S labeling with KS specific sulfotransferases, CHST1 and CHST6, followed by Recombinant F. keratolyticus Endo-beta-Galactosidase digestion, which also suggested that KS accounts for majority of the mass of this product.

References

  1. Funderburgh, J.L. (2000) Glycobiology 10:951.
  2. Suzuki, M. (1939) J. Biochem. 30:185.
  3. Meyer,K. et al. (1953) J. Biol. Chem. 205:611.
  4. Amado M. et al. (1999). Biochim. Biophys. Acta 1473:35.
  5. Lee P. L. et al. (2009) Glycobiology 19:655.
  6. Torii T. et al. (2000) Glycobiology 10:203.
  7. Oeben,M. et al. (1987) Biochem. J. 248:85.

Additional Keratan Sulfate Proteoglycans Products

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