Recombinant Bacteria Flagellin TLR5 Mutant His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18159
Key Product Details
Product Specifications
Description
Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and fused at the N-terminus to a His-tag.
Source: E. coli
Source: E. coli
NCBI: NP_460912
Purity
>95% pure by SDS-PAGE
Endotoxin Level
<0.01EU/ug purified protein (LAL test; Lonza).
Activity
Not detected by human and mouse TLR5 (Toll-like receptor 5). Does not activate TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
Specificity
Not detected by human and mouse TLR5 (Toll-like receptor 5).
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Bacteria Flagellin TLR5 Mutant His Protein
![Functional: Recombinant Bacteria Flagellin TLR5 Mutant His Protein [NBP3-18159]](https://resources.bio-techne.com/images/products/Recombinant-Human--Mouse-Flagellin-TLR5-Mutant-His-Protein-Functional-NBP3-18159-img0001.jpg "Functional: Recombinant Bacteria Flagellin TLR5 Mutant His Protein [NBP3-18159]")
Functional: Recombinant Bacteria Flagellin TLR5 Mutant His Protein [NBP3-18159]
Functional: Recombinant Human, Mouse Flagellin TLR5 Mutant His Protein [NBP3-18159] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.) activate TLR5 in vivo.Method: Mice WT are injected i.v. with 1ug of Flagellin (rec.) (=WT) or Flagellin (NLRC4 Mutant) (rec.). After 2 hours, levels of CCL20 in the serum are measured by ELISA. As control, WT mice are injected i.v. with 1ug of Flagellin (TLR5 Mutant) (rec.). PBS injected mice are used as negative control.Formulation, Preparation and Storage
NBP3-18159
| Formulation | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Flagellin
UniProt
Additional Flagellin Products
Product Documents for Recombinant Bacteria Flagellin TLR5 Mutant His Protein
Product Specific Notices for Recombinant Bacteria Flagellin TLR5 Mutant His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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