msyB (Thr2-Arg124) Accession # P25738	GSENLYFQGHMHHHHHH	F. keratolyticus Endo-beta -Galactosidase (Asp25-Leu422) Accession # Q9ZG90
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr2 (msyB)

Predicted Molecular Mass

59 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measured by its ability to cleave keratan sulfate.
50% cleavage of keratan sulfate (0.5 μg) is achieved using <5 ng rF.k. Endo-beta -galactosidase, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 8620-GH in the following applications:

Mass spectrometry analysis of N-glycan structures (1 Review)

Formulation, Preparation and Storage

8620-GH

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Endo-beta-Galactosidase

F. keratolyticus Endo-beta -Galactosidase hydrolyzes internal beta(1-4) galactose linkages in poly-N-acetyllactosamine [GlcNAc beta(1-3)Gal beta(1-4)] structures as well as sulfated structures such as keratan sulfate (1). It has wider substrate specificity and work more efficiently than its counterparts from E. freundii and B. fragilis (2). The enzyme may be used in fractional protein isolation (3). It may also be used for deglycosylation (4). Branching and/or fucosylation of the substrate may reduce or inhibit cleavage.

References

Leng, L. et al. (1998) Gene 222:187.
Amano. J. et al. (1991) J. Biol. Chem. 266:11461.
Maria Vistnes et al. (2014) PLoS ONE 9:e89621
Xiaoke Yin et al. (2013) Molecular & Cellular Proteomics 12:956.

Additional Endo-beta-Galactosidase Products

Product Documents for F. keratolyticus Endo-beta-Galactosidase Protein, CF

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SDS

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Product Specific Notices for F. keratolyticus Endo-beta-Galactosidase Protein, CF

For research use only

Citations
Reviews

F. keratolyticus Endo-beta-Galactosidase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8620-GH

Key Product Details

Source

Conjugate

Applications

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Product Specifications