E. coli O157:H7 NleL Catalytic Domain Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E3-250
Discontinued Product
E3-250 has been discontinued.
View all NleL products.
Key Product Details
Product Specifications
Source
E. coli-derived e. coli NleL protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
87 kDa
Activity
Escherichia coli O157:H7 recombinant NleL Catalytic Domain is a bacterial HECT class Ubiquitin Ligases (E3) that catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains. Reaction conditions will need to be optimized for each specific application. We recommend an initial E. coli O157:H7 recombinant NleL concentration of 0.1-0.5 μM.
Formulation, Preparation and Storage
E3-250
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
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| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: NleL
NleL (Non-Lee-encoded effector Ligase, also known as espX7) is an effector protein translocated into host cells via the type III secretion system of Escherichia coli O157:H7, a widespread pathogen causing severe foodborne diseases. Although the biological relevance of NleL is presently unknown, recent studies clearly demonstrate that this protein is functionally related to the HECT (Homologous to E6AP Carboxy Terminus) class E3 Ubiquitin Ligases of higher eukaryotes. NleL shows no primary sequence homology to any eukaryotic proteins, but biochemical and structural studies revealed both N-terminal and C-terminal lobes connected by a flexible linker, and a catalytic cysteine that accepts ubiquitin from E2 carrier proteins, both hallmarks of HECT domain ligases. NleL catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains. This recombinant protein contains amino acid residues 170-782 (UniProt Q8X5G6 numbering).
References
- Lin D.Y., et al. (2011) J. Biol. Chem. 286: 441-449.
- Lin D.Y., et al. (2012) Proc. Natl. Acad. Sci. 109: 1925-1930.
Long Name
EHEC Type III Effector NleL
UniProt
Product Documents for E. coli O157:H7 NleL Catalytic Domain Protein, CF
Product Specific Notices for E. coli O157:H7 NleL Catalytic Domain Protein, CF
For research use only
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