Recombinant Human Cyclin B2 His Protein

Name: Recombinant Human Cyclin B2 His Protein
Brand: Novus Biologicals
SKU: NBP1-98946

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98946

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Cyclin B2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-398 of Human Cyclin B2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cyclin B2 His Protein

SDS-PAGE: Recombinant Human Cyclin B2 His Protein [NBP1-98946]

SDS-Page: Recombinant Human Cyclin B2 Protein [NBP1-98946] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98946

Formulation	20 mM Tris-HCl buffer (pH 7.5), 10% glycerol, 0.5M NaCl, 250mM Imidazol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B2

CCNB2, also known as G2/mitotic-specific cyclin-B2, belongs to the cyclin family.CCNB2 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). Recombinant human CCNB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

CCNB2

Gene Symbol