What receptors does FGF basic bind?

Name: Bovine FGF basic/FGF2/bFGF Protein
Brand: R&D Systems
SKU: 133-FB

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Bovine FGF basic/FGF2/bFGF Protein

Catalog # 133-FB | R&D Systems, Inc. a Bio-Techne Brand

Citations (10)

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Key Product Details

Source:	Bovine Brain
Applications:	Bioactivity

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Citations (10) / Reviews / FAQs

Product Specifications for Bovine FGF basic/FGF2/bFGF Protein

Source	Bovine brain tissue-derived FGF basic/FGF2/bFGF protein
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is 0.125-0.625 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

133-FB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

133-FB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FGF basic/FGF2/bFGF

FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35 - 55% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities.

FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

Item	Value
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Bovine FGF basic/FGF2/bFGF Protein (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Bovine FGF basic/FGF2/bFGF Protein include:

Species: Newt
Sample Types: In Vivo
Applications: In Vivo

Tsissios et al. (2023-06-05), Macrophages modulate fibrosis during newt lens regeneration bioRxiv : the preprint server for biology
PMID: 37333184
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Tangeman J et al. (2022-04-19), A Stage-Specific OTX2 Regulatory Network and Maturation-Associated Gene Programs Are Inherent Barriers to RPE Neural Competency. Front Cell Dev Biol, 2022-010(0):875155.
PMID: 35517508
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

RW Scott et al. (2019-12-05), Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration Cell Stem Cell, 2019-125(6):797-813.e9.
PMID: 31809738
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay

X Gong et al. (2018-10-12), Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation J. Biol. Chem., 2018-10(0).
PMID: 30315105
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

BA Boswell et al. (2017-02-16), Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract Mol. Biol. Cell, 2017-00(0).
PMID: 28209733
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Tsai SY et al. (2011-06-01), Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells. Stem Cells, 2011-029(6):964-71.
PMID: 21563278
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Boswell BA et al. (2008-09-18), Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Dev. Biol., 2008-0324(2):202-12.
PMID: 18848538
Species: Ovine
Sample Types: Whole Cells
Applications: Bioassay

Zheng J et al. (2007-09-26), Activation of multiple signaling pathways is critical for fibroblast growth factor 2- and vascular endothelial growth factor-stimulated ovine fetoplacental endothelial cell proliferation. Biol. Reprod., 2007-078(1):143-50.
PMID: 17901071
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Horst AK et al. (2006-05-04), Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J. Clin. Invest., 2006-0116(6):1596-605.
PMID: 16680193
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Collombet JM et al. (2005-05-15), Effect of cytokine treatment on the neurogenesis process in the brain of soman-poisoned mice. Toxicology, 2005-0210(1):9-23.
PMID: 15804454

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Showing 1 - 10 of 10 citations Showing All

FAQs for Bovine FGF basic/FGF2/bFGF Protein

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.bio-techne.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Bovine FGF basic/FGF2/bFGF Protein

Catalog # 133-FB | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Bovine FGF basic/FGF2/bFGF Protein

Formulation, Preparation and Storage

Carrier Free

133-FB

Carrier

133-FB/CF

Carrier Free

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

Citations for Bovine FGF basic/FGF2/bFGF Protein (10)

FAQs for Bovine FGF basic/FGF2/bFGF Protein

Product Documents for Bovine FGF basic/FGF2/bFGF Protein

Product Datasheets

COA

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SDS

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