Recombinant Human Angiopoietin-2 His Protein

Name: Recombinant Human Angiopoietin-2 His Protein
Brand: Novus Biologicals
SKU: NBP2-77507

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77507

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

CHO

Tag

His

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Angiopoietin-2 Proteins and Enzymes »

Product Specifications

Description

A recombinant bioactive protein with a C terminal His-tag and corresponding to the amino acids 19-496 of Human Angiopoietin-2.

Source: CHO cell

Amino Acid Sequence: YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Measured by its binding ability in a functional ELISA with Human Tie-2

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Angiopoietin-2 His Protein

SDS-PAGE: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507]

SDS-Page: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] -

Bioactivity: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - Human Angiopoietin-2 is coated at 5 ug/ml (100 ul/well) and can bind Human Tie-2 in a Functional ELISA assay

Formulation, Preparation and Storage

NBP2-77507

Formulation	PBS (pH 7.4), 10% glycerol.
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol